TIRF 3 Nikon-Roper ILAS-2 PhotoAblation

TIRF Nikon Ti – Roper iLas-2 FRAP/Photoablation system

TIRF 3 Nikon-Roper ILAS-2 PhotoAblation
Kai Jiang is studying the microtubule minus end proteins in the cells

Primary tasks:

TIRF microscopy, Single Molecule Imaging, FRAP, Photo-Activation, Photo-Ablation (532 nm)

Microscope: Nikon Eclipse Ti with Perfect Focus System

Objective: Nikon Apo TIRF 100x N.A. 1.49 oil

Beamsplitter: Optosplit III (allows two or three color simultaneous imaging)

Photometrics Evolve Delta 512 EMCCD
Photometrics CoolSNAP Myo CCD

405 nm (100 mW)
445 nm (80 mW)
488 nm (150 mW)
561 nm (100 mW)
642 nm (120 mW)
Teem Photonics 532 nm (for photoablation)

FRAP/TIRF: Ilas-2 system (Roper Scientific France/ PICT-IBiSA, Institut Curie)

ET-BFP2 (49021), ET-GFP (49002), ET-mCherry (49008), ET-GFPmCherry (59022) – all Chroma

Stage: ASI motorized stage MS-2000-XY System for Inverted Microscope Nikon Te/Ti 2000

Filter Wheels: Sutter LB10-3 (excitation filter wheel with smart shutter; emission filter wheel; smart shutter for transmission light)

Heating: Tokai Hit INUBG2E-ZILCS Stage Top Incubator

Software: MetaMorph 7.8

Resolution: 65 nm in 1 pixel


Location: O-524
Contact Person: Ilya Grigoriev