TIRF 3 Nikon-Roper ILAS-2 PhotoAblation

TIRF Nikon Ti – Roper iLas-2 FRAP/Photoablation system

Kai Jiang is studying the microtubule minus end proteins in the cells

Primary tasks:
TIRF microscopy, Single Molecule Imaging, FRAP, Photo-Activation, Photo-Ablation (532 nm)

Microscope: Nikon Eclipse Ti with Perfect Focus System

Objective: Nikon Apo TIRF 100x N.A. 1.49 oil

Beamsplitter: Optosplit III (allows two or three color simultaneous imaging)

Camera:
Photometrics Evolve Delta 512 EMCCD
Photometrics CoolSNAP Myo CCD

Lasers:
Vortran Stradus 405 (405 nm / 100 mW)
Vortran Stradus 445 (445 nm / 80 mW)
Coherent OBIS 561-100LS (561 nm / 100 mW)
Vortran Stradus 488 (488 nm / 150 mW)
Vortran Stradus 642 (642 nm / 110 mW)
Teem Photonics 532 nm (for photoablation)

FRAP/TIRF: Ilas-2 system (Roper Scientific France/ PICT-IBiSA, Institut Curie)

Filters:
ET-BFP2 (49021), ET-GFP (49002), ET-mCherry (49008), ET-GFPmCherry (59022) – all Chroma

Stage: ASI motorized stage MS-2000-XY System for Inverted Microscope Nikon Te/Ti 2000

Filter Wheels: Sutter LB10-3 (excitation filter wheel with smart shutter; emission filter wheel; smart shutter for transmission light)

Heating: Tokai Hit INUBG2E-ZILCS Stage Top Incubator

Software: MetaMorph 7.8

Resolution: 65 nm in 1 pixel

Location: O-524
Contact Person: Ilya Grigoriev