Jan Andries Post: Cellular Electron Microscopy

CVResearchLab membersPublications


Jan A Postdr. Jan Andries Post
Cell Biology, Faculty of Science, Utrecht University
Kruytgebouw, room W505
Padualaan 8, 3584 CH Utrecht
The Netherlands
Fax. +31-(0)30-2533894
e-mail: J.A.Post@uu.nl


Curriculum Vitae

Jan Andries Post studied biology at Universiteit Utrecht and obtained his MSc in 1985 (cum laude). Afterwards he was appointment as a PhD student at the Chemistry faculty and at the interfaculty Institute of Molecular Biology and Medical Biotechnology, Universteit Utrecht, the Netherlands and received his PhD (cum laude) in 1989. His thesis was entitled “Lipid organization in myocardial sarcolemma and ischemia/reperfusion” and combined biochemistry on membrane lipids in the van Deenen lab (Chemistry) and electron microscopy in the lab of Verkleij (Biology). Afterwards he moved to the Cardiovascular Research Laboratory, UCLA (Los Angeles, USA), working on the role of sarcolemmal phospholipids in excitation-contraction coupling with Glenn A. Langer. In 1992 he returned to Utrecht as a research fellow of the Dutch Royal Academy of Sciences (KNAW) for a period of 5 years. In 1997 he was appointed as associate professor at Utrecht University at the Cell Biology department. In 2009 he became chair a.i. of Biomolecular Imaging, Utrecht and in 2014 he joined the Cell Biology group as an associate professor.

A pdf of a short CV can be downloaded here.



My research focuses on the application of electron microscopy (EM) in the field of Cellular Electron microscopy and specifically on correlative microscopy and 3D-EM (electron tomography and focused ion beam – scanning EM tomography). Correlative microscopy allows studying rare cellular events or specific cells themselves at high resolution. New applications clearly need new experimental protocols and approaches, which are goals of current projects.


Figure 1: Immuno-labeling for caveolin after tLCP in HUVECs, judged by different microscopic techniques. A) Immunofluorescence. Cells with a perforated nucleus (blue, with a black hole) show labeling (red) throughout the cytoplasm, while non-perforated cells (intact blue nuclei) are negative. B and C) 2D TEM micrographs of 50-nm thick sections of HUVECs, cut in parallel to the base of the cell monolayer. Numerous caveolae are present and show immuno-gold (5 nm) labeling in both chemically fixed (B) and high-pressure frozen, freeze-substituted and rehydrated (C) cells. In both cases membranes are well preserved, but in the latter the cytoplasm retained more material, showing the superior cell preservation. D) 2D TEM micrograph of a 350-nm thick section of a HUVEC, cut in perpendicular to the cell monolayer. Multiple caveolae labeled with 5-nm gold are present. The large gold particles (20 nm) are the fiducials used for alignment of the tilt series. E) Virtual slices (thickness ∼4 nm) extracted from the tomogram calculated for the area squared in (D). Asterisks are on the same XY coordinate and numbers indicate the slice location in Z . Arrow points to the same location of the plasma membrane as in (F). F) 3D model of caveolar structures present in the area squared in (D). A cluster of caveolae (yellow) invaginated from the plasma membrane (yellow, arrow) is surrounded by several gold particles (red) that label for caveolin. Some of the gold particles are orderly arranged in a curve following the curvature of the membrane (red with white lines). Scale bars: (A) 25 μm, (B–D) 200nm and (E) 50 nm. (Traffic 13: 926–933).

Current projects:
Cyttron II (www.cyttron.org), a consortium of 14 academic and industrial partners. Together we further develop and integrate bioimaging technologies and in which we focus on correlative imaging and 3D-EM.
Collaboration with OHSU, Portland, USA, a project on visualizing and studying cancer cell biology.
Safe design of QDs PhD project, a TNO sponsored PhD project in close collaboration with Andries Meijerink (UU, Debije Institute).


Figure 2: Validation of CLEM procedures for TEM analysis: OG-LDL uptake by HUVECs. Cells positive for OG-LDL are localized by laser scanning microscopy in fluorescence mode (A) that combined with transmitted light mode (B) provides the information necessary to map the position of the cells of interest. (C) Imaging under a phase contrast light microscope gives valuable extra information on the shape of the cells of interest. (D) The mapped areas can be easily found on the surface of the epon block that can be subsequently trimmed and sectioned en face. (E) Under TEM, cells of interest are first localized at low magnification. (F) The analysis at higher magnification reveals that cells positive for OG-LDL are rich in endosomes/lysosomes and lipid droplets. Squares in A–D correspond to quadrant ‘d4’ shown in Fig. 1(A3) or (A4) and to the same area imaged in (E). Asterisks in (E) and (F) point out the nucleus of the same cell. The arrow in (F) points to the multivesicular body showed in detail in the inset. Note that some figures were electronically flipped in order to facilitate recognition of the cells of interest through the complete procedure. Scale bars: (A)–(D), 100 μm; (E), 30 μm; (F), 1 μm; (F) inset, 100 nm. (Journal of Microscopy, 237, 208–220).


Lab members

Karin Vocking (Technical assistant) C.E.M.Vocking@uu.nl
Elleke van Harten (Ph.D. student) E.J.vanHarten@uu.nl




Sam. F.B. van Beuningen, Lena Will, Martin Harterink, Anaël Chazeau, Eljo Y. van Battum, Cátia P. Frias, Mariella A. M. Franker, Eugene A. Katrukha, Riccardo Stucchi,  Karin Vocking, Ana T. Antunes, Lotte Slender, Sofia Doulkeridou, Peter Sillevis Smitt, A.F. Maarten Altelaar, Jan A. Post, Anna Akhmanova, R. Jeroen Pasterkamp, Lukas C. Kapitein, Esther de Graaff and Casper C. Hoogenraad. TRIM46 controls neuronal polarity and axon specification by driving the formation of parallel microtubule arrays. Neuron: 88:1208-1226 (2015).

Sophie Gallier, Karin Vocking, Jan Andries Post, Bert Van De Heijning, Dennis Acton, Eline M. Van Der Beek, Ton Van Baalen. A novel infant milk formula concept: Mimicking the human milk fat globule structure. Colloids and Surfaces B: Biointerfaces 136, 329–339 (2015).

Inge van den Berg, Chantal Boonacker, Goniil Dilaver, Rolf Groenwold, Annemarie Heersche, Kim van den Heuvel, Hanna van Keulen, Toon de Kroon, Karin Scager & Jan Andries Post. Cijfers in de master meester: hoe consistent is de beoordeling van stages en scripties bij de Graduate School of Life Sciences (UU)?  Tijdschrift voor Hoger Onderwijs 33: 68 (2015)


Kleinloog R, Korkmaz E, Zwanenburg JJ, Kuijf HJ, Visser F, Blankena R, Post JA, Ruigrok YM, Luijten PR, Regli L, Rinkel GJ, Verweij BH. Visualization of the  aneurysm wall: a 7.0-tesla magnetic resonance imaging study. Neurosurgery. 2014 Dec;75(6):614-22; discussion 622. doi: 10.1227/NEU.0000000000000559. PubMed PMID: 25255252. 

Braakhuis HM, Gosens I, Krystek P, Boere JA, Cassee FR, Fokkens PH, Post JA, van Loveren H, Park MV. Particle size dependent deposition and pulmonary inflammation after short-term inhalation of silver nanoparticles. Part Fibre Toxicol. 2014 Sep 17;11(1):49. doi: 10.1186/s12989-014-0049-1. PubMed PMID: 25227272; PubMed Central PMCID: PMC4410796.  

Jiménez N, Post JA. A novel permeabilization protocol to obtain intracellular  3D immunolabeling for electron tomography. Methods Mol Biol. 2014;1174:285-95. doi: 10.1007/978-1-4939-0944-5_20. PubMed PMID: 24947390. 


Allijn IE, Leong W, Tang J, Gianella A, Mieszawska AJ, Fay F, Ma G, Russell S, Callo CB, Gordon RE, Korkmaz E, Post JA, Zhao Y, Gerritsen HC, Storm G, Thran A, Proksa R, Daerr H, Fuster V, Fisher EA, Fayad ZA, Mulder WJ, Cormode DP.Gold Nanocrystal Labeling Allows Low Density Lipoprotein Imaging From The Subcellular To Macroscopic Level. ACS Nano. 26:9761-9770 (2013)

Marijn Kuijpers, Vera van Dis, Elize D Haasdijk, Martin Harterink, Karin Vocking, Jan A Post, Wiep Scheper, Casper C Hoogenraad, Dick Jaarsma. Amyotrophic lateral sclerosis (ALS)-associated VAPB-P56S inclusions represent an ER quality control compartment. Acta Neuropathologica Communications 1:24 (2013).

B. Lubelli, D.A.M. de Winter, J.A. Post, R.P.J. van Heesa, M.R. Drury. Cryo-FIB–SEM and MIP study of porosity and pore size distribution of bentonite and kaolin at different moisture contents. Applied Clay Science 80, 358-365 (2013).

Matthijs De Winter Rob J Mesman, Michael F Hayles; Chris T Schneijdenberg, Cliff Mathisen, Jan A Post. In-situ Integrity Control of frozen-hydrated, vitreous lamellas prepared by the cryo-Focused Ion Beam-Scanning Electron Microscope. J. Struct. Biol. 183:11-18 (2013).

Jan Hilhorst, D. A. Matthijs de Winter, Joost Wolters, Jan Andries Post, and Andrei V. Petukhov Defect Engineering in Sedimentary Colloidal Crystals to produce Photonic Crystals. Langmuir, 29:10011-10018 (2013).

Peter T. Witte, Susan Boland, Fiona Kirby, Ramon van Maanen, Bas F. Bleeker, Matthijs de Winter, Jan A. Post, John W. Geus, Peter H. Berben. NanoSelectTM Pd catalysts: what causes the high selectivity of these supported colloidal catalysts in alkyne semi-hydrogenation? Chem Cat Chem 5: 582-587 (2013).

Nuria Jiménez; Vincent J Krouwer and Jan A Post. A new, rapid and reproducible method to obtain high quality endothelium in vitro. Cytotechnology 65: 1-14 (2013).


V.J. Krouwer, Liesbeth Hekking, Miriam Langelaar-Makkinje, Elsa Regan-Klapisz and Jan Andries Post. Endothelial cell senescence is associated with disrupted cell-cell junctions and increased monolayer permeability. Vascular Cell 4:12 (2012).

Eline Hendrike Verbon, Jan Andries Post, Johannes Boonstra. The Influence of Reactive Oxygen Species on Cell Cycle Progression in Mammalian Cells. Gene 511: 1-6 (2012)

N. Jiménez and J.A.Post. Intracellular 3D immuno-labeling, for electron tomography, after targeted laser cell perforation.Traffic, 13:926-933 (2012).

Marie Renault, Ria Tommassen-van Boxtel, Martine P. Bos, Jan Andries Post, Jan Tommassen and Marc Baldus. Cellular solid-state Nuclear Magnetic Resonance spectroscopy. PNAS USA, 109:4863-4868 (2012).

Jeanine Inamuco , Andreas K.J. Veenendaal, Sara A. Burt, J.A. Post , Johanna L.M. Tjeerdsma-van Bokhoven, Henk P. Haagsman, Edwin J.A.Veldhuizen. Sub-lethal Levels of Carvacrol Reduce Salmonella Typhimurium Motility and Invasion of porcine Epithelial Cells. Vet. Mic.127:200-207 (2012).

M.N.Lebbink, L.H.P.Hekking, W.J.C.Geerts, and J.A.Post. D-CAT: Density and Clustering Annotation Tool for Three Dimensional Electron Microscopic Volume. J.Struct.Biol. 177:571-577 (2012).


Willie J.C. Geerts, Karin Vocking, Natasja Schoonen, Lindsay Haarbosch, Elly van Donselaar, Elza Regan-Klapisz and Jan Andries Post. Cobblestone HUVECs: A Human Model System for studying Primary Ciliogenesis. J. Struct. Biol. 176:350-359 (2011).

Luis R. Aramburo, Lukasz Karwacki, Pablo Cubillas, Shunsuke Asahina, D. A. Matthijs de Winter, Martyn R. Drury, Inge L.C. Buurmans, Eli Stavitski, Davide Mores, Marco Daturi, Philippe Bazin, Paul Dumas, Fréderic Thibault-Starzyk, Norma Kahn, Jan A. Post, Simon R. Bare, Michael W. Anderson, Osamu Terasaki,k and Bert M. Weckhuysen. Porosity, Acidity and Reactivity of Dealuminated Zeolite ZSM-5 at the Single Particle Level: Influence of the Zeolite Architecture. Chemistry A European Journal. 17:13773-13781 (2011).

D.A.M. de Winter, M.N. Lebbink, D.F. Wiggers de Vries, J.A. Post and M.R. Drury. FIB-SEM cathodoluminescence Tomography: Practical and Theoretical Considerations. J. Microscopy, 243:315-26 (2011).

Lukasz Karwacki, D.A. Matthijs de Winter, Luis R. Aramburo, Misjael N. Lebbink, Jan Andries Post, Martyn R. Drury, and Bert M. Weckhuysen. Architecture Dependent Distribution of Mesopores in Steamed Zeolite Crystals as Visualized by FIB-SEM Tomography. Angewandte Chemie, 50:1294-1298 (2011). 

Ilse Gosens, Jan Andries Post, Liset J.J. de la Fonteyne, Eugene H.J.M. Jansen, John W. Geus, Flemming R. Cassee and Wim H. de Jong. Impact of agglomeration state of nano- and submicron sized gold particles on pulmonary inflammation. Particle and Fibre Toxicology, 7:37-48 (2011). 

Evisa Bood-Gjini, Liesbeth H. Hekking, Axel Küchler, Erno Wienholds, Jan Andries Post and Stefan Schulte-Merker. Zebrafish Tie-2 shares redundant role with Tie-1 in heart development and regulates vessel integrity. Disease Models & Mechanisms 4: 57-66 (2011).


Torjus Skajaa, Yiming V. Zhao, Dave J. van den Heuvel, Hans C. Gerritsen, David P. Cormode, Rolf Koole, Matti M van Schooneveld, Jan Andries Post, Edward A. Fisher, Zahi A. Fayad, Celso de Mello Donega, Andries Meijerink, Willem J.M. Mulder. Quantum dot and Cy5.5 labeled nanoparticles to investigate lipoprotein biointeractions via Förster resonance energy transfer. Nano Letters. 10, 5131–5138 (2010). 

Misjaël N. Lebbink, Nuria Jiménez, Karin Vocking, Liesbeth H. Hekking, Arie J. Verkleij, Jan A. Post. Spiral coating of the endothelial caveolar membranes as revealed by electron tomography and template matching. Traffic. 11: 138-150 (2010). 

Nuria Jiménez, Elly G. van Donselaar, D.A. Matthijs de Winter, Karin Vocking, Arie J. Verkleij and Jan A. Post. Correlative light and electron microscopy, by TEM and FIB-SEM, using gridded Aclar as substrate for cell monolayer culture. J. Microscopy. 237:208-220 (2010). 

Gosens I, Leseman DLAC, Fonteyne LJJ de la, Lankveld DPK, Geus JW, Post JA, Jansen EHJM, Boere AJF, Cassee FR, Jong WH de. Impact of agglomeration state of gold particles on pulmonary toxicity. RIVM Letter Report 340370002/2010 (2010).


Grundmann S, Schirmer SH, Hekking LH, Post JA, Ionita MG, de Groot D, van Royen N, van den Berg B, Vink H, Moser M, Bode C, de Kleijn D, Pasterkamp G, Piek JJ, Hoefer P. Endothelial glycocalyx dimensions are reduced in growing collateral arteries and modulate leukocyte adhesion in arteriogenesis. J Cell Mol Med. 13(9B):3463-3474 (2009). 

Elsa Regan-Klapisz, Vincent Krouwer, Miriam Langelaar-Makkinje, Laxman Nallan, Michael Gelb, Hans Gerritsen, Arie J. Verkleij and Jan Andries Post. Golgi-associated cPLA2 regulates Endothelial Cell-Cell Junction Integrity by controlling the trafficking of transmembrane junction proteins Mol. Biol. Cell 20: 4225-4234 (2009). 

L.H.P. Hekking, M.N. Lebbink, D.A.M. de Winter, C.T.W.M. Schneijdenberg, C.M. Brand, B.M. Humbel, A.J. Verkleij and J.A. Post. Focused ion beam-scanning electron microscope: exploring large volumes of atherosclerotic tissue J. Microscopy 235: 336-347 (2009). 

Misjaël N. Lebbink, Elly van Donselaar, Bruno Humbel, Louis Hertzberger, Jan Andries Post and Arie Verkleij. Induced Membrane Domains as Visualized by Electron Tomography and Template Matching. J. Struct. Biol. 166: 156-161 (2009). 

Jakub Rohlena, Oscar L.Volger, Jaap D. van Buul, Liesbeth H.P. Hekking, Janine van Gils, Peter Bonta, Jan Andries Post, Peter L. Hordijk and Anton J.G. Horrevoets. Endothelial CD81 is a Marker of Early Human Artherosclerotic Plaques and Facilitates Monocyte Adhesion Independent of Inflammation. Cardiovasc. Res. 81: 187-196 (2009). 

J.A.Post. Electron Microscopy and Cardiovascular Disease. In 3D EM in Life Sciences, Chapter 6.07; Eds. Orlova and Verkleij, European 3D EM Network of Excellence DVD. 

Nuria Jiménez, Karin Vocking, Elly van Donselaar, Bruno H. Humbel, Jan A. Post and Arie J. Verkleij. Tannic Acid-Mediated Osmium Impregnation After Freeze-Substitution: A Strategy to Enhance Membrane Contrast For Electron Tomography Of Non-Post-Stained Plastic Sections. J. Structural. Biol. 166: 103-106 (2009). 

Rinse Klooster, Quint le Duc, Michael R. Eman, C. Theo Verrips and Jan A. Post. Differential ER resident protein expression in HeLa cells during ER stress: Selection and application of KDEL specific VHH antibodies. J. Immunological Methods 342:1-12 (2009). 

FAC Wiegant, S Surinova, E Ytsma, M Langelaar-Makkinje, G Wikman and JA Post. Plant adaptogens increase lifespan and stress resistance in C.elegans. Biogerontoloy. 10: 27-42 (2009)


Oosterling, S. J., van der Bij, G. J., Bogels, M., Raa, S. T., Post, J. A., Meijer, G. A., Beelen, R. H., and Egmond, M. V. Anti-beta1 Integrin Antibody Reduces Surgery-Induced Adhesion of Colon Carcinoma Cells to Traumatized Peritoneal Surfaces. Ann Surg 247: 85-94 (2008). 

Wiegant, F.A.C., Limandjaja, G., de Poot, S.A.H., Bayda, L.A., Vorontsova, O.N., Zenina, T.A., Langelaar-Makkinje, M, Post, J.A. and Wikman, G. Plant Adaptogens Activate Cellular Adaptive Mechanisms by Causing Mild Damage. In: Adaptation Biology and Medicine: Health Potentials. Eds. Lukyanova, L., Takeda, N. and Singal, P.K., Narosa Publishers, New Delhi, India, Vol. 5, 319-332 (2008).


A.E. Yakushevska, M.N. Lebbink, W.J.C. Geerts, L. Spek, E.G. van Donselaar, K.A. Jansen, B.M. Humbel, J.A. Post, A.J.Verkleij and A.J. Koster. STEM tomography in cell biology. Journal of Structural Biology 159: 381–391 (2007).

Rinse Klooster, Bram T. H. Maassen, Jord C. Stam, Pim W. Hermans, Mark R. ten Haaft, Frank J.M. Detmers, Hans J. de Haard, Jan A. Post and C. Theo Verrips. Improved anti-IgG and HSA affinity ligands: Clinical application of VHH antibody technology. J. Immunological Methods 324:1-12 (2007).


David A. Ishola, Jr., Jan Andries Post, Mirjan M. van Timmeren, Stephan J. L. Bakker, Roel Goldschmeding, Hein A. Koomans, Branko Braam, and Jaap A. Joles. In proximal tubular cells, aggravation of albumin-induced oxidative stress by bound fatty acids is linked with altered mitochondrial redox balance. Kidney Int, 70:724-731 (2006).

Branko Braam; Miriam Langelaar-Makkinje; Arie Verkleij; Hans Bluyssen; Theo Verrips; Hein A. Koomans; Jaap A. Joles, DVM, PhD; Jan Andries Post. Anti-oxidant sensitivity of donor age-related gene expression in cultured fibroblasts. European J Pharmacology, 542: 154-161 (2006).

Michael R. Eman, Elsa Regan-Klapisz, Martijn W. H. Pinkse, Inge M. Koop, Johan Haverkamp, Albert J.R. Heck, Arie J. Verkleij and Jan A. Post. Protein expression dynamics during replicative senescence of endothelial cells studied by two-dimensional difference in-gel electrophoresis. Electrophoresis 27: 1669-1682 (2006).

L.M. Bevers, B. Braam, P.M. de Bree, J.A. Post, A.J. van Zonneveld, T.J. Rabelink, H.A. Koomans, M.C. Verhaar, J.A. Joles. Tetrahydrobiopterin-dependent NO synthase uncoupling in cells expressing high levels of endothelial NO synthase. Hypertension 47: 87-94 (2006).


J. Op den Buijs, M. Musters, T. Verrips, J.A. Post, G.B. Braam, N. van Riel. Mathematical modeling of vascular endothelial layer maintenance: the role of endothelial cell division, progenitor cell homing and telomere shortening. Am. J. Physiol. 287:H2651-H2658 (2004).

G.B. Braam, R. de Roos, A. Dijk, P. Boer, J.A. Post, P. Kemmeren, F. Holstege, H.A.R. Bluyssen and H. Koomans. Nitric oxide donor induces temporal and dose-dependent reduction of gene expression in human endothelial cells. Am. J. Physiol. 287: H1977-H1986 (2004)

J. Boonstra and J.A. Post. Molecular events associated with reactive oxygen species and cell cycle progression in mammalian cells. Gene 337: 1-13 (2004)

G.P.C.Drummen, B.M. Gadella, J.A. Post and J.F.H.M. Brouwers. Mass spectrometric characterization of the oxidation of the fluorescent lipid peroxidation reporter molecule C11-BODIPY 581/591 in homogeneous solution and endogenously stressed Rat-1 fibroblasts. Free Rad. Biol. Med. 36: 1635-1644 (2004).

G.S.A.T. van Rossum, G.P.C. Drummen, A.J. Verkleij, J.A. Post and J. Boonstra. Activation of cytosolic phospholipase A2 in Her14 fibroblasts by hydrogen peroxide: a p42/44MAPK dependent and a phosphorylation independent mechanism. Biochim. Biophys. Acta, 1636: 183-195 (2004).

G. P.C. Drummen, M. Makkinje. A. J. Verkleij, J.A.F. Op den Kamp and J.A. Post. Attenuation of lipid peroxidation by antioxidants in rat-1 fibroblasts: Comparison of the lipid peroxidation reporter molecules cis-parinaric acid and C11-BODIPY581/591 in a biological setting. Biochim. Biophys. Acta, 1636: 136-150 (2004).


D. van der Vlies, M. Makkinje, A. Jansens, I. Braakman, A.J. Verkleij, K.W.A. Wirtz and J.A. Post. Oxidation of ER resident proteins upon oxidative stress. Effects of altering cellular redox/antioxidant status and implications for protein maturation. Antioxid Redox Signal 5:381-387 (2003).

D. van der Vlies , J. Woudenberg, J.A. Post. Protein oxidation in aging: endoplasmic reticulum as a target. Amino Acids 25: 397-407 (2003). 

C. Martinez-Munoz and J.A. Post. The role of reactive oxygen species in G1 progression. In: Regulation of mitose to G1 transition. Ed. J. Boonstra, Landes Biosci. (2003).


S. Bapat, J.A. Post, B. Braam, R. Goldschmeding, H.A. Koomans, A.J. Verkleij and J.A. Joles. Visualizing tubular lipid peroxidation in intact renal tissue in hypertensive rats. J. Am. Soc. Nephrol. J Am Soc Nephrol 12: 2990-2996 (2002).

C. Martínez Muñoz, L.A. van Meeteren, J.A. Post, A.J. Verkleij, C.T. Verrips and J. Boonstra. Hydrogen peroxide inhibits cell cycle progression by inhibition of the spreading of mitotic cells. Free Rad Biol Med 33: 1061-1072 (2002).

D. van der Vlies, E.H.W. Pap, J.A. Post, J.E. Celis and K.W.A. Wirtz. Endoplasmic reticulum resident proteins of normal human dermal fibroblasts are the major targets for oxidative stress induced by hydrogen peroxide. Biochem. J. 366: 825-830 (2002).

G.P.C. Drummen, L.C.M van Liebergen, J.A.F. Op den Kamp and J.A. Post. C11-BODIPY581/591, an oxidation sensitive fluorescent lipid peroxidation probe: (Micro)spectroscopic characterization and validation of methodology. Free Rad Biol Med 33: 473-490 (2002). 

R. Steenbergen, J.A.F. Op den Kamp, A.J. Verkleij and J.A. Post. Onderzoek naar de veranderingen in de celmembraan tijdens ischemie en reperfusie. Cordiaal 1: 18-21 (2002).


C.T. Verrips, M. Warmoeskerken and J.A.Post. General introduction to the importance of genomics to food biotechnology and nutrition. Current Opinion in Biotechnology 12: 483-487 (2001).

R. de Wit, M. Makkinje, J. Boonstra, A.J. Verkleij and J.A. Post. Hydrogen peroxide reversibly inhibits Epidermal Growth Factor (EGF) receptor internalization and coincident ubiquitination of the EGF receptor and Eps15. FASEB J. 15: 306-308 (2001).

C. Martinez-Muñoz, J.A. Post, C.T. Verrips, A.J. Verkleij and J. Boonstra. Effect of H2O2 on Cyclin D expression in fibroblasts. Cell. Mol. Life Sci. 58: 990-996 (2001).

S. Bapat, A.J. Verkleij and J.A. Post. Peroxynitrite activates mitogen-activated protein kinase (MAPK) via a MEK-independent pathway: A role for protein kinase C. FEBS letters 499:21-26 (2001).