Category: BIC

Testing The Nanoimager (ONI, Oxford Nanoimaging)

2017.05.17 Testing The Nanoimager (ONI, Oxford Nanoimaging)
http://www.oxfordni.com/

Cell Biology Utrecht University, Room Z-512… Read more

Posted in Activities, BIC

Testing Live-SR (Roper)

2017.03.21 Testing Live-SR (Roper)
Live Super-Resolution Confocal Spinning Disc Microscopy

Cell Biology Utrecht University, Room O-524… Read more

Posted in Activities, BIC

Testing Nikon BioStation IMq

2016.10.20-27 Testing Nikon BioStation IMq

20161020-27-testing-nikon-biostation-imq-1
Sanne Remmelzwaal  and Wilco Nijenhuis are discussing the performance of the BioStation.

20161020-27-testing-nikon-biostation-imq-2
U2OS cells at 20x, phase contrast, chromatin in green, (H2B-YFP) and tubulin in red (mCherry-tubulin)

 

Cell Biology Utrecht University, Room N-532… Read more

Posted in Activities, BIC

Testing Biotek Cytation 5

2016.09.05-08 Testing Biotek Cytation 5

20160905-08 Testing Biotek Cytation 5 Image 1

20160905-08 Testing Biotek Cytation 5 Image 2
Wound healing assay of HeLa cells over 2 days, phase contrast, stitched from 3 images, 4x

20160905-08 Testing Biotek Cytation 5 Image 3
mCherry-tubulin and H2B-YFP in U2OS cells at 20x

 

 

Cell Biology Utrecht University, Room N-532… Read more

Posted in Activities, BIC

Digital inverted fluorescent microscope AMG EVOS

Digital inverted fluorescent microscope AMG EVOS
Qingyang Liu and Sanne Remmelzwaal are checking the HeLa cells transfected with wild-type Rab8 protein.

Primary tasks: Cell Culture Observation

Microscope: AMG EVOS

Objective:

  • LPlan PH2 4x/0.13 phase-contrast
  • Plan Fluor 10x/0.3
  • Plan Fluor 20x/0.5
  • LPlan PH2 20x/0.40 phase-contrast

Epi-fluorescence: GFP, … Read more

Posted in Equipment

Upright fluorescence microscope Nikon Ni-E

Upright FL microscope Nikon Eclipse Ni
Jingchao Wu is studying

Primary tasks: Imaging of fixed samples

Microscope: Nikon Eclipse Ni-E

Objective:

  • Plan Apo Lambda 100x N.A. 1.45 oil
  • Plan Apo Lambda 60x N.A. 1.40 oil
  • Plan Fluor 40x N.A. 1.30 oil
  • Plan Fluor 20x N.A. 0.75
… Read more
Posted in Equipment

Super-Resolution STORM/PALM custom made system

Primary tasks:
3D Single Molecule Localization Microscopy using Adaptive Optics

Microscope: Nikon Eclipse Ti with Perfect Focus System 3

Objectives:

Nikon Apo TIRF 100x/1.49 NA oil 
Nikon PLAN FLUOR 10x/0.3 NA air

Camera: 2 x Hamamatsu Flash 4.0v2

Lasers:
Omicron … Read more

Posted in Equipment

TIRF Nikon Ti – Roper iLas-2 FRAP/Photoablation

TIRF 3 Nikon-Roper ILAS-2 PhotoAblation
Kai Jiang is studying the microtubule minus end proteins in the cells

Primary tasks:
TIRF microscopy, Single Molecule Imaging, FRAP, Photo-Activation, Photo-Ablation (532 nm)

Microscope: Nikon Eclipse Ti with Perfect Focus System

Objective: Apo TIRF 100x / 1.49, oil, WD=0.09-0.16 … Read more

Posted in Equipment