Lukas Kapitein: Biophysics

CVResearch – Lab membersPublications


Prof. Dr. Lukas C. Kapitein
Cell Biology, Neurobiology and Biophysics, Faculty of Science, Utrecht University
Kruytgebouw, room Z510
Padualaan 8, 3584 CH Utrecht
The Netherlands
Tel. +31-(0)30-2533629


Curriculum Vitae

Lukas Kapitein studied Physics at the VU University in Amsterdam, where he also received his PhD in Biophysics in 2007. His postdoctoral training in Neurobiology was at the Erasmus Medical Center, funded by an ALW-VENI grant from the Netherlands Organisation for Scientific Research (NWO) and an Erasmus MC Fellowship. He started as an group leader at Utrecht University in 2011 and became full professor in Molecular and Cellular Biophysics in 2018. In 2013, he received a Starting Grant from the European Research Council (ERC) and a VIDI fellowship from the Netherlands Organisation for Scientific Research (NWO). In 2018, he received an ERC Consolidator Grant.

Lukas Kapitein co-manages the Gravitation project IMAGINE! (Innovative Microscopy and Guidance of cells In their Native Environment), led by Anna Akhmanova (2022-2032).

A pdf of a short CV can be found here.


Research summary

Our goal is to obtain a physical understanding of the mechanisms by which cells establish and maintain their precise shape and intracellular organization. We focus on the cytoskeleton, a mechanical network of biopolymers and associated proteins that gives the cell its shape and strength. Motor proteins can move over these biopolymers to deliver cargo to specific subcellular compartments or to reorganize the fibers themselves.

Our research currently concentrates on the cytoskeleton of neurons, the brain cells whose complex spatial architecture and polarized organization into axons and dendrites are crucial for proper functioning. Obtaining physical insight in the mechanisms that establish polarity, polarized transport and dendrite maturation is important, as neurodegenerative diseases often correlate with altered morphology and distorted intracellular transport. Our research is organized around two central ambitions:

  • Finding the road map: we employ or develop advanced optical methods to resolve the precise intracellular architecture and dynamics of the neuronal cytoskeleton. This includes mapping the distribution of microtubule orientations, their modifications, and their interacting proteins using super-resolution microscopy (STED, Localization Microscopy, Expansion Microscopy).


  • Finding the traffic rules: we employ or develop controlled intracellular assays to probe the cooperative dynamics of specific motor-cargo assemblies. This will reveal how specific motor proteins interact with the neuronal cytoskeleton to drive selective transport to dendrites and axons, as wells as in and out of dendritic spines.


To achieve these goals, we combine protein engineering, advanced (super-resolution) microscopy techniques and mathematical modeling.

Interested in participating? We are always interested in connecting with enthusiastic researchers that are also fascinated by the topics we study. Feel free to reach out to us by writing to or check the ‘positions’ section.


Lab members


Scientific Staff  
Wilco Nijenhuis
Eugene Katrukha
Janine McCaughey
Wendy van Straaten
Ernest van der Wee (Microscopy specialist)
Josiah Passmore
Alfredo Rates Alfredo Rates
PhD students:  
Hugo Damstra
Malina Iwanski
Daphne Jurriens
Giel Korsten
Albert Serweta
Thijs Makaske
Jasper Schelt



For the most up-to-date list of publication click here


Damstra HGJ, Mohar B, Eddison M, Akhmanova A, Kapitein LC, Tillberg PW. Ten-fold Robust Expansion Microscopy. Bio Protoc. 2023 Jun 20;13(12):e4698. doi: 10.21769/BioProtoc.4698. PMID: 37397797; PMCID: PMC10308184.

Truong MA, Cané-Gasull P, de Vries SG, Nijenhuis W, Wardenaar R, Kapitein LC, Foijer F, Lens SM. A kinesin-based approach for inducing chromosome-specific mis-segregation in human cells. EMBO J. 2023 May 15;42(10):e111559. doi: 10.15252/embj.2022111559. Epub 2023 Apr 11. PMID: 37038978; PMCID: PMC10183822.

Iwanski MK, Kapitein LC. Cellular cartography: Towards an atlas of the neuronal microtubule cytoskeleton. Front Cell Dev Biol. 2023 Mar 22;11:1052245. doi: 10.3389/fcell.2023.1052245. PMID: 37035244; PMCID: PMC10073685.

Jansen KI, Iwanski MK, Burute M, Kapitein LC. A live-cell marker to visualize the dynamics of stable microtubules throughout the cell cycle. J Cell Biol. 2023 May 1;222(5):e202106105. doi: 10.1083/jcb.202106105. Epub 2023 Mar 2. PMID: 36880745; PMCID: PMC9998657.

Deguchi T, Iwanski MK, Schentarra EM, Heidebrecht C, Schmidt L, Heck J, Weihs T, Schnorrenberg S, Hoess P, Liu S, Chevyreva V, Noh KM, Kapitein LC, Ries J. Direct observation of motor protein stepping in living cells using MINFLUX. Science. 2023 Mar 10;379(6636):1010-1015. doi: 10.1126/science.ade2676. Epub 2023 Mar 9. PMID: 36893247; PMCID: PMC7614483.


Damstra HGJ, Mohar B, Eddison M, Akhmanova A, Kapitein LC, Tillberg PW. Visualizing cellular and tissue ultrastructure using Ten-fold Robust Expansion Microscopy (TREx). Elife. 2022 Feb 18;11:e73775. doi: 10.7554/eLife.73775. PMID: 35179128; PMCID: PMC8887890.

Meiring JCM, Grigoriev I, Nijenhuis W, Kapitein LC, Akhmanova A. Opto-katanin, an optogenetic tool for localized, microtubule disassembly. Curr Biol. 2022 Nov 7;32(21):4660-4674.e6. doi: 10.1016/j.cub.2022.09.010. Epub 2022 Sep 28. PMID: 36174574.

Burute M, Jansen KI, Mihajlovic M, Vermonden T, Kapitein LC. Local changes in microtubule network mobility instruct neuronal polarization and axon specification. Sci Adv. 2022 Nov 4;8(44):eabo2343. doi: 10.1126/sciadv.abo2343. Epub 2022 Nov 4. PMID: 36332030; PMCID: PMC9635826.

Hung ST, Llobet Rosell A, Jurriens D, Siemons M, Soloviev O, Kapitein LC, Grußmayer K, Neukomm LJ, Verhaegen M, Smith C. Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains. Front Neurosci. 2022 Oct 6;16:954949. doi: 10.3389/fnins.2022.954949. PMID: 36278016; PMCID: PMC9583434.

Amatngalim GD, Rodenburg LW, Aalbers BL, Raeven HH, Aarts EM, Sarhane D, Spelier S, Lefferts JW, Silva IA, Nijenhuis W, Vrendenbarg S, Kruisselbrink E, Brunsveld JE, van Drunen CM, Michel S, de Winter-de Groot KM, Heijerman HG, Kapitein LC, Amaral MD, van der Ent CK, Beekman JM. Measuring cystic fibrosis drug responses in organoids derived from 2D differentiated nasal epithelia. Life Sci Alliance. 2022 Aug 3;5(12):e202101320. doi: 10.26508/lsa.202101320. PMID: 35922154; PMCID: PMC9351388.

Siemons ME, Kapitein LC, Stallinga S. Axial accuracy in localization microscopy with 3D point spread function engineering. Opt Express. 2022 Aug 1;30(16):28290-28300. doi: 10.1364/OE.461750. PMID: 36299028.

Akhmanova A, Kapitein LC. Mechanisms of microtubule organization in differentiated animal cells. Nat Rev Mol Cell Biol. 2022 Aug;23(8):541-558. doi: 10.1038/s41580-022-00473-y. Epub 2022 Apr 5. PMID: 35383336.

Chen F, Wu J, Iwanski MK, Jurriens D, Sandron A, Pasolli M, Puma G, Kromhout JZ, Yang C, Nijenhuis W, Kapitein LC, Berger F, Akhmanova A. Self-assembly of pericentriolar material in interphase cells lacking centrioles. Elife. 2022 Jul 5;11:e77892. doi: 10.7554/eLife.77892. PMID: 35787744; PMCID: PMC9307276.

Hung ST, Cnossen J, Fan D, Siemons M, Jurriens D, Grußmayer K, Soloviev O, Kapitein LC, Smith CS. SOLEIL: single-objective lens inclined light sheet localization microscopy. Biomed Opt Express. 2022 May 10;13(6):3275-3294. doi: 10.1364/BOE.451634. PMID: 35781973; PMCID: PMC9208595.



Janssen AFJ, Korsten G, Nijenhuis W, Katrukha EA, Kapitein LC. Direct observation of aggregate-triggered selective autophagy in human cells. J Cell Sci. 2021 Oct 1;134(19):jcs258824. doi: 10.1242/jcs.258824. Epub 2021 Oct 6. PMID: 34447998; PMCID: PMC8520732.

Passmore JB, Nijenhuis W, Kapitein LC. From observing to controlling: Inducible control of organelle dynamics and interactions. Curr Opin Cell Biol. 2021 Aug;71:69-76. doi: 10.1016/ Epub 2021 Mar 8. PMID: 33706236.

Katrukha EA, Jurriens D, Salas Pastene DM, Kapitein LC. Quantitative mapping of dense microtubule arrays in mammalian neurons. Elife. 2021 Jul 27;10:e67925. doi: 10.7554/eLife.67925. PMID: 34313224; PMCID: PMC8416025.

Özkan N, Koppers M, van Soest I, van Harten A, Jurriens D, Liv N, Klumperman J, Kapitein LC, Hoogenraad CC, Farías GG. ER – lysosome contacts at a pre-axonal region regulate axonal lysosome availability. Nat Commun. 2021 Jul 23;12(1):4493. doi: 10.1038/s41467-021-24713-5. PMID: 34301956; PMCID: PMC8302662.

Buijs RR, Hummel JJA, Burute M, Pan X, Cao Y, Stucchi R, Altelaar M, Akhmanova A, Kapitein LC, Hoogenraad CC. WDR47 protects neuronal microtubule minus ends from katanin-mediated severing. Cell Rep. 2021 Jul 13;36(2):109371. doi: 10.1016/j.celrep.2021.109371. PMID: 34260930.

Siemons ME, Hanemaaijer NAK, Kole MHP, Kapitein LC. Robust adaptive optics for localization microscopy deep in complex tissue. Nat Commun. 2021 Jun 7;12(1):3407. doi: 10.1038/s41467-021-23647-2. PMID: 34099685; PMCID: PMC8184833.

Gros OJ, Damstra HGJ, Kapitein LC, Akhmanova A, Berger F. Dynein self-organizes while translocating the centrosome in T-cells. Mol Biol Cell. 2021 Apr 19;32(9):855-868. doi: 10.1091/mbc.E20-10-0668. Epub 2021 Mar 10. PMID: 33689395; PMCID: PMC8108531.

Jurriens D, van Batenburg V, Katrukha EA, Kapitein LC. Mapping the neuronal cytoskeleton using expansion microscopy. Methods Cell Biol. 2021;161:105-124. doi: 10.1016/bs.mcb.2020.04.018. Epub 2020 Jun 3. PMID: 33478685.




Hooikaas PJ, Damstra HG, Gros OJ, van Riel WE, Martin M, Smits YT, van Loosdregt J, Kapitein LC, Berger F, Akhmanova A. Kinesin-4 KIF21B limits microtubule growth to allow rapid centrosome polarization in T cells. Elife. 2020 Dec 21;9:e62876. doi: 10.7554/eLife.62876. PMID: 33346730; PMCID: PMC7817182.

Serra-Marques A, Martin M, Katrukha EA, Grigoriev I, Peeters CA, Liu Q, Hooikaas PJ, Yao Y, Solianova V, Smal I, Pedersen LB, Meijering E, Kapitein LC, Akhmanova A. Concerted action of kinesins KIF5B and KIF13B promotes efficient secretory vesicle transport to microtubule plus ends. Elife. 2020 Nov 11;9:e61302. doi: 10.7554/eLife.61302. PMID: 33174839; PMCID: PMC7710357.

Müller-Deku A, Meiring JCM, Loy K, Kraus Y, Heise C, Bingham R, Jansen KI, Qu X, Bartolini F, Kapitein LC, Akhmanova A, Ahlfeld J, Trauner D, Thorn-Seshold O. Photoswitchable paclitaxel-based microtubule stabilisers allow optical control over the microtubule cytoskeleton. Nat Commun. 2020 Sep 15;11(1):4640. doi: 10.1038/s41467-020-18389-6. PMID: 32934232; PMCID: PMC7493900.

Nijenhuis W, van Grinsven MMP, Kapitein LC. An optimized toolbox for the optogenetic control of intracellular transport. J Cell Biol. 2020 Apr 6;219(4):e201907149. doi: 10.1083/jcb.201907149. PMID: 32328628; PMCID: PMC7147098.

Rodríguez-García R, Volkov VA, Chen CY, Katrukha EA, Olieric N, Aher A, Grigoriev I, López MP, Steinmetz MO, Kapitein LC, Koenderink G, Dogterom M, Akhmanova A. Mechanisms of Motor-Independent Membrane Remodeling Driven by Dynamic Microtubules. Curr Biol. 2020 Mar 23;30(6):972-987.e12. doi: 10.1016/j.cub.2020.01.036. Epub 2020 Feb 7. PMID: 32032506; PMCID: PMC7090928.

Cao Y, Lipka J, Stucchi R, Burute M, Pan X, Portegies S, Tas R, Willems J, Will L, MacGillavry H, Altelaar M, Kapitein LC, Harterink M, Hoogenraad CC. Microtubule Minus-End Binding Protein CAMSAP2 and Kinesin-14 Motor KIFC3 Control Dendritic Microtubule Organization. Curr Biol. 2020 Mar 9;30(5):899-908.e6. doi: 10.1016/j.cub.2019.12.056. Epub 2020 Feb 20. PMID: 32084403; PMCID: PMC7063570.

Rai A, Liu T, Glauser S, Katrukha EA, Estévez-Gallego J, Rodríguez-García R, Fang WS, Díaz JF, Steinmetz MO, Altmann KH, Kapitein LC, Moores CA, Akhmanova A. Taxanes convert regions of perturbed microtubule growth into rescue sites. Nat Mater. 2020 Mar;19(3):355-365. doi: 10.1038/s41563-019-0546-6. Epub 2019 Dec 9. PMID: 31819210.

Siemons M, Cloin BMC, Salas DM, Nijenhuis W, Katrukha EA, Kapitein LC. Comparing strategies for deep astigmatism-based single-molecule localization microscopy. Biomed Opt Express. 2020 Jan 10;11(2):735-751. doi: 10.1364/BOE.382023. PMID: 32133221; PMCID: PMC7041481.



Fréal A, Rai D, Tas RP, Pan X, Katrukha EA, van de Willige D, Stucchi R, Aher A, Yang C, Altelaar AFM, Vocking K, Post JA, Harterink M, Kapitein LC, Akhmanova A, Hoogenraad CC. Feedback-Driven Assembly of the Axon Initial Segment. Neuron. 2019 Oct 23;104(2):305-321.e8. doi: 10.1016/j.neuron.2019.07.029.

Burute M, Kapitein LC. Cellular Logistics: Unraveling the Interplay Between Microtubule Organization and Intracellular Transport. Annu Rev Cell Dev Biol. 2019 Oct 6;35:29-54. doi: 10.1146/annurev-cellbio-100818-125149.

Harterink M, Vocking K, Pan X, Soriano Jerez EM, Slenders L, Fréal A, Tas RP, van de Wetering WJ, Timmer K, Motshagen J, van Beuningen SFB, Kapitein LC, Geerts WJC, Post JA, Hoogenraad CC. TRIM46 Organizes Microtubule Fasciculation in the Axon Initial Segment. J Neurosci. 2019 Jun 19;39(25):4864-4873. doi: 10.1523/JNEUROSCI.3105-18.2019.

Stanganello E, Zahavi EE, Burute M, Smits J, Jordens I, Maurice MM, Kapitein LC, Hoogenraad CC. Wnt Signaling Directs Neuronal Polarity and Axonal Growth. iScience. 2019 Mar 29;13:318-327. doi: 10.1016/j.isci.2019.02.029.

Hooikaas PJ, Martin M, Mühlethaler T, Kuijntjes GJ, Peeters CAE, Katrukha EA, Ferrari L, Stucchi R, Verhagen DGF, van Riel WE, Grigoriev I, Altelaar AFM, Hoogenraad CC, Rüdiger SGD, Steinmetz MO, Kapitein LC, Akhmanova A. MAP7 family proteins regulate kinesin-1 recruitment and activation. J Cell Biol. 2019 Apr 1;218(4):1298-1318. doi: 10.1083/jcb.201808065.


Tas RP, Chen CY, Katrukha EA, Vleugel M, Kok M, Dogterom M, Akhmanova A, Kapitein LC. Guided by Light: Optical Control of Microtubule Gliding Assays. Nano Lett. 2018 Dec 12;18(12):7524-7528. doi: 10.1021/acs.nanolett.8b03011.

Janssen AFJ, Katrukha EA, van Straaten W, Verlhac P, Reggiori F, Kapitein LC. Probing aggrephagy using chemically-induced protein aggregates. Nat Commun. 2018 Oct 12;9(1):4245. doi: 10.1038/s41467-018-06674-4.

Harterink M, Edwards SL, de Haan B, Yau KW, van den Heuvel S, Kapitein LC, Miller KG, Hoogenraad CC. Local microtubule organization promotes cargo transport in C. elegans dendrites. J Cell Sci. 2018 Oct 22;131(20). pii: jcs223107. doi: 10.1242/jcs.223107.

Sun F, Adrian M, Beztsinna N, van den Dikkenberg JB, Maas-Bakker RF, van Hasselt PM, van Steenbergen MJ, Su X, Kapitein LC, Hennink WE, van Nostrum CF. Influence of PEGylation of Vitamin-K-Loaded Mixed Micelles on the Uptake by and Transport through Caco-2 Cells. Mol Pharm. 2018 Sep 4;15(9):3786-3795. doi: 10.1021/acs.molpharmaceut.8b00258.

Cunha-Ferreira I, Chazeau A, Buijs RR, Stucchi R, Will L, Pan X, Adolfs Y, van der Meer C, Wolthuis JC, Kahn OI, Schätzle P, Altelaar M, Pasterkamp RJ, Kapitein LC, Hoogenraad CC. The HAUS Complex Is a Key Regulator of Non-centrosomal Microtubule Organization during Neuronal Development. Cell Rep. 2018 Jul 24;24(4):791-800. doi: 10.1016/j.celrep.2018.06.093.

Tas RP, Kapitein LC. Exploring cytoskeletal diversity in neurons. Science. 2018 Jul 20;361(6399):231-232. doi: 10.1126/science.aat5992.

Kahn OI, Schätzle P, van de Willige D, Tas RP, Lindhout FW, Portegies S, Kapitein LC, Hoogenraad CC. APC2 controls dendrite development by promoting microtubule dynamics. Nat Commun. 2018 Jul 17;9(1):2773. doi: 10.1038/s41467-018-05124-5.

Aher A, Kok M, Sharma A, Rai A, Olieric N, Rodriguez-Garcia R, Katrukha EA, Weinert T, Olieric V, Kapitein LC, Steinmetz MO, Dogterom M, Akhmanova A. CLASP Suppresses Microtubule Catastrophes through a Single TOG Domain. Dev Cell. 2018 Jul 2;46(1):40-58.e8. doi: 10.1016/j.devcel.2018.05.032.

Schätzle P, Esteves da Silva M, Tas RP, Katrukha EA, Hu HY, Wierenga CJ, Kapitein LC, Hoogenraad CC. Activity-Dependent Actin Remodeling at the Base of Dendritic Spines Promotes Microtubule Entry. Curr Biol. 2018 Jul  9;28(13):2081-2093.e6. doi: 10.1016/j.cub.2018.05.004.

Tas RP, Bos TGAA, Kapitein LC. Purification and Application of a Small Actin Probe for Single-Molecule Localization Microscopy. Methods Mol Biol. 2018;1665:155-171. doi: 10.1007/978-1-4939-7271-5_9.


Tas RP, Chazeau A, Cloin BMC, Lambers MLA, Hoogenraad CC, and Kapitein LC. Differentiation between Oppositely Oriented Microtubules Controls Polarized Neuronal Transport. Neuron, 2017; 96 (6): 1264-1271 e5.

Janssen AFJ, Tas RP, van Bergeijk P, Oost R, Hoogenraad CC, and Kapitein LC. Myosin-V Induces Cargo Immobilization and Clustering at the Axon Initial Segment. Front Cell Neurosci, 2017; 11: 260.

Adrian M, Kusters R, Storm C, Hoogenraad CC, and Kapitein LC. Probing the Interplay between Dendritic Spine Morphology and Membrane-Bound Diffusion. Biophys J, 2017; 113 (10): 2261-2270.

Cloin BMC, De Zitter E, Salas D, Gielen V, Folkers GE, Mikhaylova M, Bergeler M, Krajnik B, Harvey J, Hoogenraad CC, Van Meervelt L, Dedecker P, and Kapitein LC. Efficient switching of mCherry fluorescence using chemical caging. Proc Natl Acad Sci U S A, 2017; 114 (27): 7013-7018.

Tortosa E, Adolfs Y, Fukata M, Pasterkamp RJ, Kapitein LC, and Hoogenraad CC. Dynamic Palmitoylation Targets MAP6 to the Axon to Promote Microtubule Stabilization during Neuronal Polarization. Neuron, 2017; 94 (4): 809-825 e7.

Gumy LF, Katrukha EA, Grigoriev I, Jaarsma D, Kapitein LC, Akhmanova A, and Hoogenraad CC. MAP2 Defines a Pre-axonal Filtering Zone to Regulate KIF1- versus KIF5-Dependent Cargo Transport in Sensory Neurons. Neuron, 2017; 94 (2): 347-362 e7.

Harterink M, da Silva ME, Will L, Turan J, Ibrahim A, Lang AE, van Battum EY, Pasterkamp RJ, Kapitein LC, Kudryashov D, Barres BA, Hoogenraad CC, and Zuchero JB. DeActs: genetically encoded tools for perturbing the actin cytoskeleton in single cells. Nat Methods, 2017; 14 (5): 479-482.

Adrian M, Nijenhuis W, Hoogstraaten RI, Willems J, and Kapitein LC. A Phytochrome-Derived Photoswitch for Intracellular Transport. ACS Synth Biol, 2017; 6 (7): 1248-1256.

Katrukha EA, Mikhaylova M, van Brakel HX, van Bergen En Henegouwen PM, Akhmanova A, Hoogenraad CC, and Kapitein LC. Probing cytoskeletal modulation of passive and active intracellular dynamics using nanobody-functionalized quantum dots. Nat Commun, 2017; 8: 14772.

van Riel WE, Rai A, Bianchi S, Katrukha EA, Liu Q, Heck AJ, Hoogenraad CC, Steinmetz MO, Kapitein LC, and Akhmanova A. Kinesin-4 KIF21B is a potent microtubule pausing factor. Elife, 2017; 6.


Kaplan M, Narasimhan S, de Heus C, Mance D, van Doorn S, Houben K, Popov-Celeketic D, Damman R, Katrukha EA, Jain P, Geerts WJ, Heck AJ, Folkers GE, Kapitein LC, Lemeer S, van Bergen En Henegouwen PM, and Baldus M. EGFR Dynamics Change during Activation in Native Membranes as Revealed by NMR. Cell, 2016. 167(5): 1241-1251 e11.

Noordstra I, Liu Q, Nijenhuis W, Hua S, Jiang K, Baars M, Remmelzwaal S, Martin M, Kapitein LC, and Akhmanova A. Control of apico-basal epithelial polarity by the microtubule minus-end-binding protein CAMSAP3 and spectraplakin ACF7. J Cell Sci, 2016. 129(22): 4278-4288.

Liu Q, Liu F, Yu KL, Tas R, Grigoriev I, Remmelzwaal S, Serra-Marques A, Kapitein LC, Heck AJ, and Akhmanova A. MICAL3 Flavoprotein Monooxygenase Forms a Complex with Centralspindlin and Regulates Cytokinesis. J Biol Chem, 2016. 291(39): 20617-29.

Doodhi H, Prota AE, Rodriguez-Garcia R, Xiao H, Custar DW, Bargsten K, Katrukha EA, Hilbert M, Hua S, Jiang K, Grigoriev I, Yang CP, Cox D, Horwitz SB, Kapitein LC, Akhmanova A, and Steinmetz MO. Termination of Protofilament Elongation by Eribulin Induces Lattice Defects that Promote Microtubule Catastrophes. Curr Biol, 2016. 26(13): 1713-21.

Franker MA, Esteves da Silva M, Tas RP, Tortosa E, Cao Y, Frias CP, Janssen AF, Wulf PS, Kapitein LC, and Hoogenraad CC. Three-Step Model for Polarized Sorting of KIF17 into Dendrites. Curr Biol, 2016. 26(13): 1705-12.

Kevenaar JT, Bianchi S, van Spronsen M, Olieric N, Lipka J, Frias CP, Mikhaylova M, Harterink M, Keijzer N, Wulf PS, Hilbert M, Kapitein LC, de Graaff E, Ahkmanova A, Steinmetz MO, and Hoogenraad CC. Kinesin-Binding Protein Controls Microtubule Dynamics and Cargo Trafficking by Regulating Kinesin Motor Activity. Curr Biol, 2016. 26(7): 849-61.

Harterink M, van Bergeijk P, Allier C, de Haan B, van den Heuvel S, Hoogenraad CC, and Kapitein LC. Light-controlled intracellular transport in Caenorhabditis elegans. Curr Biol, 2016. 26(4): R153-4.

Kuijpers M, van de Willige D, Freal A, Chazeau A, Franker MA, Hofenk J, Rodrigues RJ, Kapitein LC, Akhmanova A, Jaarsma D, and Hoogenraad CC. Dynein Regulator NDEL1 Controls Polarized Cargo Transport at the Axon Initial Segment. Neuron, 2016. 89(3): 461-71.

Yau KW, Schatzle P, Tortosa E, Pages S, Holtmaat A, Kapitein LC, and Hoogenraad CC. Dendrites In Vitro and In Vivo Contain Microtubules of Opposite Polarity and Axon Formation Correlates with Uniform Plus-End-Out Microtubule Orientation. J Neurosci, 2016. 36(4): 1071-85.

Lipka J, Kapitein LC, Jaworski J, and Hoogenraad CC. Microtubule-binding protein doublecortin-like kinase 1 (DCLK1) guides kinesin-3-mediated cargo transport to dendrites. EMBO J, 2016. 35(3): 302-18.

Chazeau A, Katrukha EA, Hoogenraad CC, and Kapitein LC. Studying neuronal microtubule organization and microtubule-associated proteins using single molecule localization microscopy. Methods Cell Biol, 2016. 131: 127-49.

Schatzle P, Kapitein LC, and Hoogenraad CC. Live imaging of microtubule dynamics in organotypic hippocampal slice cultures. Methods Cell Biol, 2016. 131: 107-26.

van Bergeijk P, Hoogenraad CC, and Kapitein LC. Right Time, Right Place: Probing the Functions of Organelle Positioning. Trends Cell Biol, 2016. 26(2): 121-34.


van Beuningen SF, Will L, Harterink M, Chazeau A, van Battum EY, Frias CP, Franker MA, Katrukha EA, Stucchi R, Vocking K, Antunes AT, Slenders L, Doulkeridou S, Sillevis Smitt P, Altelaar AF, Post JA, Akhmanova A, Pasterkamp RJ, Kapitein LC, de Graaff E, Hoogenraad CC. TRIM46 Controls Neuronal Polarity and Axon Specification by Driving the Formation of Parallel Microtubule Arrays. Neuron. 2015 Dec 16;88(6):1208-26. doi: 10.1016/j.neuron.2015.11.012. Epub 2015 Dec 6. PubMed PMID: 26671463.

Esteves da Silva M, Adrian M, Schätzle P, Lipka J, Watanabe T, Cho S, Futai K, Wierenga CJ, Kapitein LC, Hoogenraad CC. Positioning of AMPA Receptor-Containing Endosomes Regulates Synapse Architecture. Cell Rep. 2015 Nov 3;13(5):933-43. doi: 10.1016/j.celrep.2015.09.062. Epub 2015 Oct 22. PubMed PMID:   26565907.

van Bergeijk P, Hoogenraad CC, Kapitein LC. Right Time, Right Place: Probing the Functions of Organelle Positioning. Trends Cell Biol. 2015 Nov 2. pii: S0962-8924(15)00188-9. doi: 10.1016/j.tcb.2015.10.001. PubMed PMID:   26541125.

Mikhaylova M, Cloin BM, Finan K, van den Berg R, Teeuw J, Kijanka MM, Sokolowski M, Katrukha EA, Maidorn M, Opazo F, Moutel S, Vantard M, Perez F, van Bergen en Henegouwen PM, Hoogenraad CC, Ewers H, Kapitein LC. Resolving bundled microtubules using anti-tubulin nanobodies. Nat Commun. 2015 Aug 11;6:7933. doi: 10.1038/ncomms8933. PubMed PMID:   26260773.

Kapitein LC, Hoogenraad CC. Building the Neuronal Microtubule Cytoskeleton. Neuron. 2015 Aug 5;87(3):492-506. doi: 10.1016/j.neuron.2015.05.046. PubMed PMID:   26247859.

Van Bergeijk P, Adrian M, Hoogenraad CC, Kapitein LC. Optogenetic control of organelle transport and positioning. Nature. 2015 Jan 7. doi: 10.1038/nature14128. PMID: 25561173


Adrian M, Kusters R, Wierenga CJ, Storm C, Hoogenraad CC, Kapitein LC. Barriers in the brain: resolving dendritic spine morphology and compartmentalization. Front Neuroanat. 2014 Dec 4;8:142. doi: 10.3389/fnana.2014.00142. PMID: 25538570

Cloin BM, Hoogenraad CC, Mikhaylova M, and Kapitein LC. Single Molecule Localization Microscopy to Study Neuronal Microtubule Organization. Immunocytochemistry and Related Techniques, Neuromethods, 2014 vol. 101, DOI 10.1007/978-1-4939-2313-7_21

Yau KW, van Beuningen SF, Cunha-Ferreira I, Cloin BM, van Battum EY, Will L, Schätzle P, Tas RP, van Krugten J, Katrukha EA, Jiang K, Wulf PS, Mikhaylova M, Harterink M, Pasterkamp RJ, Akhmanova A, Kapitein LC, Hoogenraad CC. Microtubule minus-end binding protein CAMSAP2 controls axon specification and dendrite development. Neuron. 2014 Jun 4;82(5):1058-73. doi: 10.1016/j.neuron.2014.04.019. PMID: 24908486

Gumy LF, Katrukha EA, Kapitein LC, Hoogenraad CC. New insights into mRNA trafficking in axons. Dev Neurobiol. 2014 Mar;74(3):233-44. doi: 10.1002/dneu.22121. Epub 2013 Sep 30. Review. PubMed PMID: 23959656

Doodhi H, Katrukha EA, Kapitein LC, Akhmanova A. Mechanical and geometrical constraints control kinesin-based microtubule guidance. Curr Biol. 2014 Feb 3;24(3):322-8. doi: 10.1016/j.cub.2014.01.005. PMID: 24462000


Kusters R, Kapitein LC, Hoogenraad CC, and Storm C. Shape-induced asymmetric diffusion in dendritic spines allows efficient synaptic AMPA receptor trapping. Biophys J. 2013 Dec 17, 105(12): 2743-50. PMID: 24359746.

Gumy LF, Chew DJ, Tortosa E, Katrukha EA, Kapitein LC, Tolkovsky AM, Hoogenraad CC, Fawcett JW. The Kinesin-2 Family Member KIF3C Regulates Microtubule Dynamics and Is Required for Axon Growth and Regeneration. J Neurosci. 2013 Jul 10;33(28):11329-45. PMID: 23843507

Mohan R, Katrukha EA, Doodhi H, Smal I, Meijering E, Kapitein LC, Steinmetz MO, Akhmanova A. End-binding proteins sensitize microtubules to the action of microtubule-targeting agents. Proc Natl Acad Sci U S A. 2013 May 28;110(22):8900-5. PMID: 23674690

Kapitein LC, van Bergeijk P, Lipka J, Keijzer N, Wulf PS, Katrukha EA, Akhmanova A, Hoogenraad CC. Myosin-V opposes microtubule-based cargo transport and drives directional motility on cortical actin. Curr Biol. 2013 May 6;23(9):828-34. PMID:23602478 

Van Spronsen M, Mikhaylova M, Lipka J, Schlager MA, van den Heuvel DJ, Kuijpers M, Wulf PS, Keijzer N, Demmers J, Kapitein LC, Jaarsma D, Gerritsen HC, Akhmanova A, Hoogenraad CC.TRAK/Milton motor-adaptor proteins steer mitochondrial trafficking to axons and dendrites. Neuron. 2013 Feb 6;77(3):485-502. PMID: 23395375


Kapitein LC, Mackintosh FC, Hoogenraad CC. De verkeersregels in hersencellen. Nederlands Tijdschrift voor Natuurkunde 2012


Kapitein LC, Yau KW, Gouveia SM, van der Zwan WA, Wulf PS, Keijzer N, Demmers J, Jaworski J, Akhmanova A, Hoogenraad CC.NMDA receptor activation suppresses microtubule growth and spine entry. J Neurosci. 2011 Jun 1;31(22):8194-209. PMID:21632941

Hellal F, Hurtado A, Ruschel J, Flynn KC, Laskowski CJ, Umlauf M, Kapitein LC, Strikis D, Lemmon V, Bixby J, Hoogenraad CC, Bradke F. Microtubule stabilization reduces scarring and causes axon regeneration after spinal cord injury. Science. 2011 Feb 18;331(6019):928-31. PMID: 21273450

Kapitein LC, Hoogenraad CC. Which way to go? Cytoskeletal organization and polarized transport in neurons. Mol Cell Neurosci. 2011 Jan;46(1):9-20. PMID: 20817096


Kapitein LC, Yau KW, Hoogenraad CC. Microtubule dynamics in dendritic spines. Methods Cell Biol. 2010;97:111-32. PMID: 20719268

Montenegro Gouveia S, Leslie K, Kapitein LC, Buey RM, Grigoriev I, Wagenbach M, Smal I, Meijering E, Hoogenraad CC, Wordeman L, Steinmetz MO, Akhmanova A. In vitro reconstitution of the functional interplay between MCAK and EB3 at microtubule plus ends. Curr Biol. 2010 Oct 12;20(19):1717-22. PMID: 20850319

Kapitein LC, Schlager MA, van der Zwan WA, Wulf PS, Keijzer N, Hoogenraad CC. Probing intracellular motor protein activity using an inducible cargo trafficking assay. Biophys J. 2010 Oct 6;99(7):2143-52. PMID: 20923648

Lakämper S, Thiede C, Düselder A, Reiter S, Korneev MJ, Kapitein LC, Peterman EJ, Schmidt CF. The effect of monastrol on the processive motility of a dimeric kinesin-5 head/kinesin-1 stalk chimera. J Mol Biol. 2010 May 28;399(1):1-8. PMID: 20227420

Schlager MA, Kapitein LC, Grigoriev I, Burzynski GM, Wulf PS, Keijzer N, de Graaff E, Fukuda M, Shepherd IT, Akhmanova A, Hoogenraad CC. Pericentrosomal targeting of Rab6 secretory vesicles by Bicaudal-D-related protein 1 (BICDR-1) regulates neuritogenesis. EMBO J. 2010 May 19;29(10):1637-51. PMID: 20360680

Kapitein LC, Schlager MA, Kuijpers M, Wulf PS, van Spronsen M, MacKintosh FC, Hoogenraad CC. Mixed microtubules steer dynein-driven cargo transport into dendrites. Curr Biol. 2010 Feb 23;20(4):290-9. PMID: 20137950

Hoogenraad CC, Popa I, Futai K, Martinez-Sanchez E, Wulf PS, van Vlijmen T, Dortland BR, Oorschot V, Govers R, Monti M, Heck AJ, Sheng M, Klumperman J, Rehmann H, Jaarsma D, Kapitein LC, van der Sluijs P. Neuron specific Rab4 effector GRASP-1 coordinates membrane specialization and maturation of recycling endosomes. PLoS Biol. 2010 Jan 19;8(1):e1000283. PMID: 20098723

Stiess M, Maghelli N, Kapitein LC, Gomis-Rüth S, Wilsch-Bräuninger M, Hoogenraad CC, Tolić-Nørrelykke IM, Bradke F. Axon extension occurs independently of centrosomal microtubule nucleation. Science. 2010 Feb 5;327(5966):704-7. PMID: 20056854



Jaworski J, Kapitein LC, Gouveia SM, Dortland BR, Wulf PS, Grigoriev I, Camera P, Spangler SA, Di Stefano P, Demmers J, Krugers H, Defilippi P, Akhmanova A, Hoogenraad CC. Dynamic microtubules regulate dendritic spine morphology and synaptic plasticity. Neuron. 2009 Jan 15;61(1):85-100. PMID: 19146815


Van den Wildenberg SM, Tao L, Kapitein LC, Schmidt CF, Scholey JM, Peterman EJ. The homotetrameric kinesin-5 KLP61F preferentially crosslinks microtubules into antiparallel orientations. Curr Biol. 2008 Dec 9;18(23):1860-4. PMID: 19062285

Kapitein LC, Janson ME, van den Wildenberg SM, Hoogenraad CC, Schmidt CF, Peterman EJ. Microtubule-driven multimerization recruits ase1p onto overlapping microtubules. Curr Biol. 2008 Nov 11;18(21):1713-7. PMID: 18976915

Kapitein LC, Kwok BH, Weinger JS, Schmidt CF, Kapoor TM, Peterman EJ. Microtubule cross-linking triggers the directional motility of kinesin-5. J Cell Biol. 2008 Aug 11;182(3):421-8. PMID: 18678707

Kapitein LC, Peterman EJ. Walking hand-in-hand: single molecule experiments and the kinesin superfamily. Single Molecule Biology 2008: 35-59. Edited by A. Knight.


Verbrugge S, Kapitein LC, Peterman EJ. Kinesin moving through the spotlight: single-motor fluorescence microscopy with submillisecond time resolution. Biophys J. 2007 Apr 1;92(7):2536-45. PMID: 17237204

Kwok BH, Kapitein LC, Kim JH, Peterman EJ, Schmidt CF, Kapoor TM. Allosteric inhibition of kinesin-5 modulates its processive directional motility. Nat Chem Biol. 2006 Sep;2(9):480-5. PMID: 16892050

Kapitein LC, Peterman EJ, Kwok BH, Kim JH, Kapoor TM, Schmidt CF. The bipolar mitotic kinesin Eg5 moves on both microtubules that it crosslinks. Nature. 2005 May 5;435(7038):114-8. PMID: 15875026

Dijk MA, Kapitein LC, Mameren J, Schmidt CF, Peterman EJ. Combining optical trapping and single-molecule fluorescence spectroscopy: enhanced photobleaching of fluorophores. J Phys Chem B. 2004 May 20;108(20):6479-84. PMID: 18950137

Peterman EJ, Dijk MA, Kapitein LC, Schmidt CF. Extending the bandwidth of optical-tweezers interferometry. Rev of Sci Instr 2003: 77: 3246-3249