Category: Cell Biology

Inverted Microscope Nikon TS-100

Inverted Microscope Nikon TS-100
Benjamin Bouchet is checking the growth of the colony of the primary mammary cells

Primary tasks: Cell Culture Observation

Microscope: Nikon TS-100

Objective:
    Plan Fluor 4x N.A. 0.13 phase-contrast
    ADL 10x N.A. 0.25 apodized phase contrast… Read more

Posted in BIC, Equipment

Upright fluorescence microscope Nikon Eclipse 80i

Upright fluorescence microscope Nikon Eclipse 80i
Helma van Riel and master student Kim Boonekamp are checking microtubule binding of kinesin Kif21A mutants


Primary tasks:
Imaging of fixed samples

Microscope: Nikon Eclipse 80i

Objective:

  • Plan Apo VC 100x N.A. 1.40 oil
  • Plan Apo VC 60x N.A. 1.40
Read more
Posted in BIC, Equipment

Plasma Cleaner

Plasma Cleaner 1

Plasma Cleaner 2
Eugene Katrukha and Bas Cloin are cleaning the cover glasses for the single molecule imaging

Primary tasks:
• Cleaning the cover glasses for the single molecule imaging

Model: Harrick Plasma Cleaner PDC-002

Includes a 6″ diameter x 6.5″ length Pyrex … Read more

Posted in BIC, Equipment

Wide-field Nikon Ti “PEX”-scope

Wide-field Nikon Ti PEXscope 1

Sam van Beuningen and Mariella Franker are studying the molecular motors using inducible PEX assay

Primary tasks:
• Long-term imaging
• DIC
• Phase-Contrast

Microscope: Nikon Ti

Fluorescence Filters: ET-EBFP2 (49021), ET-GFP (49002), ET-mCherry (49008), ET-Qdot 625 – all Chroma… Read more

Posted in BIC, Equipment

TIRF Nikon Ti – Roper iLas FRAP/Photoablation system

TIRF Nikon Ti Roper iLas FRAPPhotoablation system
Andrea Marques and Stine Morthorst are studying the exocytosis of Rab6 vesicles


Primary tasks:
TIRF microscopy, Single Molecule Imaging, FRAP, Photo-Activation, Photo-Ablation (532 nm)

Microscope: Nikon Eclipse Ti with Perfect Focus System

Objective: Apo TIRF 100x / 1.49, oil, WD=0.09-0.16 … Read more

Posted in BIC, Equipment

Confocal Spinning Disc 1 Nikon Ti – Yokogawa X1 – Gataca iLAS FRAP/Photoablation system

Confocal Yokogawa Spinning Disc X1 Nikon Ti Roper iLas FRAPPhotoablation system
Microscopy facility manager Ilya Grigoriev, in front of Spinning Disc microscope

Primary tasks:

  • Confocal Spinning Disc microscopy
  • 3D imaging
  • FRAP
  • Photo-Activation
  • Photo-Ablation (355 nm)

Microscope: Eclipse Ti-E with PFS (Nikon, Japan)

Stage: MS-2000-XYZ with Piezo Top Plate (ASI, USA)

Piezo-Z: Read more

Posted in BIC, Equipment

Confocal Laser Scanning microscope Zeiss LSM700

Confocal Laser Scanning microscope Carl Zeiss LSM700 1

Confocal Laser Scanning microscope Carl Zeiss LSM700 2
Marijn Kuijpers and Laura Gumy are studying microtubules in the growth cone during axon regeneration

 

Primary tasks:

  • Confocal microscopy
  • 3D imaging
  • FRAP

Microscope: AxioObserver Z1

Objectives:

     EC Plan-Neofluar 10x/0.3 (WD=5.2mm)
     Plan-Apochromat 20x/0.5 (WD=2mm)
     EC Plan-Neofluar 40x/1.30 … Read more

Posted in BIC, Equipment

Activities

When   What   Where
2018.11.22   Testing ZEISS Celldiscoverer 7   Zeiss, Breda
2018.11.15   Testing STED microscope STEDYCON (Abberior Instruments)   Cell Biology Utrecht University
2018.06.21   Nikon A1R HD resonant confocal event   Nikon, Amsterdam
2017.11.09  
Read more
Posted in Activities, BIC