Inverted Microscope Nikon TS-100
Benjamin Bouchet is checking the growth of the colony of the primary mammary cells
Primary tasks: Cell Culture Observation
Microscope: Nikon TS-100
Objective:
Plan Fluor 4x N.A. 0.13 phase-contrast
ADL 10x N.A. 0.25 apodized phase contrast… Read more
Upright fluorescence microscope Nikon Eclipse 80i
Helma van Riel and master student Kim Boonekamp are checking microtubule binding of kinesin Kif21A mutants
Primary tasks: Imaging of fixed samples
Microscope: Nikon Eclipse 80i
Objective:
- Plan Apo VC 100x N.A. 1.40 oil
- Plan Apo VC 60x N.A. 1.40
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Plasma Cleaner
Eugene Katrukha and Bas Cloin are cleaning the cover glasses for the single molecule imaging
Primary tasks:
• Cleaning the cover glasses for the single molecule imaging
Model: Harrick Plasma Cleaner PDC-002
Includes a 6″ diameter x 6.5″ length Pyrex … Read more
Wide-field Nikon Ti “PEX”-scope
Sam van Beuningen and Mariella Franker are studying the molecular motors using inducible PEX assay
Primary tasks:
• Long-term imaging
• DIC
• Phase-Contrast
Microscope: Nikon Ti
Fluorescence Filters: ET-EBFP2 (49021), ET-GFP (49002), ET-mCherry (49008), ET-Qdot 625 – all Chroma… Read more
TIRF Nikon Ti – Roper iLas FRAP/Photoablation system
Andrea Marques and Stine Morthorst are studying the exocytosis of Rab6 vesicles
Primary tasks:
TIRF microscopy, Single Molecule Imaging, FRAP, Photo-Activation, Photo-Ablation (532 nm)
Microscope: Nikon Eclipse Ti with Perfect Focus System
Objective: Apo TIRF 100x / 1.49, oil, WD=0.09-0.16 … Read more
Confocal Spinning Disc 1 Nikon Ti – Yokogawa X1 – Gataca iLAS FRAP/Photoablation system
Microscopy facility manager Ilya Grigoriev, in front of Spinning Disc microscope
Primary tasks:
- Confocal Spinning Disc microscopy
- 3D imaging
- FRAP
- Photo-Activation
- Photo-Ablation (355 nm)
Microscope: Eclipse Ti-E with PFS (Nikon, Japan)
Stage: MS-2000-XYZ with Piezo Top Plate (ASI, USA)
Piezo-Z: … Read more
Confocal Laser Scanning microscope Zeiss LSM700
Marijn Kuijpers and Laura Gumy are studying microtubules in the growth cone during axon regeneration
Primary tasks:
- Confocal microscopy
- 3D imaging
- FRAP
Microscope: AxioObserver Z1
Objectives:
EC Plan-Neofluar 10x/0.3 (WD=5.2mm)
Plan-Apochromat 20x/0.5 (WD=2mm)
EC Plan-Neofluar 40x/1.30 … Read more