Services

Utrecht Nanobody Facility

  • Production of immune libraries (with QVQ)
  • Selection of nanobodies that bind with high specificity and affinity to a target (usually <10 nM)
  • Selection of nanobodies from synthetic libraries
  • Production and purification at different yield using E. coli or mammalian HEK cells
  • Fusion to different tags such as myc, his, EPEA, FLAG, GFP, RFP, among other
  • Site-directed conjugation to fluorophores (Alexa, Atto, NIR dyes), biotin or nanoparticles
  • Engineering of nanobodies in different formats, including bivalent, bispecific, trimeric, biparatopic
  • Typical time lines are three months for immunization and library construction and 1-2 months for selection, though subjected to availability
  • Costs may be involved, depending on the set-up of the collaboration

Please contact us for more details.