Confocal Laser Scanning microscope Zeiss LSM880 Fast AiryScan


Boris Shneyer and Sanne Remmelzwaal are discussing microtubule nucleation in differentiated primary muscle cells.

Primary tasks:

  • Confocal microscopy
  • 3D imaging
  • SuperResolution

Microscope: inverted Axio Observer 7 SP with Definite Focus 2 (Zeiss, Germany)

Stage: scanning stage 130×100 STEP Set LSM (Märzhäuser, Germany)

Piezo-Z: inlegframe Z-piezo WSB 500 Universal (Märzhäuser, Germany)

Objectives:

    Plan-Apochromat 20x/0.8 WD=0.55mm, Air (Zeiss, Germany)
    Plan-Apochromat 25x/0.8 WD=0.57mm, Water, Oil or Gly Immersion (Zeiss, Germany)
    Plan-Apochromat 63x/1.2 WD=0.49mm, Oil or Gly Immersion (Zeiss, Germany)
    C-Apochromat 63x/1.15, WD=0.60mm, Water Immersion (Zeiss, Germany)
    Alpha Plan-APO 100x/1.46 WD=0.10mm, Oil Immersion (Zeiss, Germany)

Light source (laser lines): 405nm, Laser Argon Multiline (445/488/514), 561nm and 633 nm (Laser Rack LSM880) (Zeiss, Germany)

Light source (epifluorescence): HXP120V (Zeiss, Germany)

Detectors:

    Spectral detector three multialkali PMT (MA-PMT) (Scan module LSM880, Zeiss, Germany)
    AiryScan detector (Airyscan module for LSM, Zeiss, Germany)

DIC: available

Temperature control and CO2: S1 (Pecon, Germany)

Software: ZEN black v2.3 (Zeiss, Germany)

Installation: 2018

 

Location: O-518
Contact Person: Ruben Schmidt / Ilya Grigoriev