Andor benchtop confocal BC43

Primary task: Long-term spinning disk confocal imaging of living cells and organoids.

Illumination: Brightfield and differential phase contrast transmitted white light, wide-field epifluorescence, Borealis™-illuminated multipoint confocal with 405/488/561/638 nm excitation lasers.

Objectives:
2x Plan UW Air 0.06NA 7.5WD
10X Plan Apo LD Air 0.45NA 4.0WD
20X Plan Apo LD Air 0.8NA 0.8WD
40X Plan Apo LD Air 0.95NA 0.21WD
60X Plan Apo LD Oil 1.42NA 0.15WD

Emission filters: DAPI, GFP, mCherry, CY5

Camera: 4.1 MP (6.5μm pixel, 16-bit) sCMOS camera up to 82% QE. 18.4 mm field of view.

Samples: multiwell plates, slides. Temperature, humidity and CO2 control

Software: Fusion BC43 acquisition software (Imaris integrated)

Installation: 2023

Location: O-519
Contact Person: Saskia Suijkerbuijk / Ruben Schmidt