Confocal Leica SP8 FLIM STED3X super-resolution

Super Resolution and confocal microscope Leica TCS SP8 STED3X SMD FLIM

Confocal Leica SP8 FLIM STED3X super-resolution
Joanna Lipka is studying the microtubules in primary hippocampal neurons  

Primary tasks:

  • STED super-resolution imaging
  • Confocal live cell microscopy
  • FLIM
  • FCS

Leica DMI 6000 CS AFC with Adaptive Focus Control
Confocal module with filter free spectral detection, 2 PMT and 3 HyD detectors
Gated and 3D STED
Single molecule TCSPC system PicoHarp 300

20x (dry) HC PL FLUOTAR 20x/ NA 0.4 CORR (working distance 7mm)
20x (oil) HC PL APO 20x/NA 0.75 IMM CORR CS2
40x (oil) HC PL APO 40x/NA 1.30 OIL CS2
63x (oil) HC PL APO 63x/NA 1.40 OIL CS2
63x (water) HC PL APO 63x/1.20 W CORR CS2
86x (water) HC PL APO 86x/NA 1.20 W motCORR STED
93x (glycerol) HC PL APO 93x/1.30 GLYC motCORR STED
100x (oil) HC PL APO 100x/1.40 STED

Laser lines
405 nm DMOD Flexible
440 pulsed FLIM
White Laser (470 670 nm)
Argon laser
STED 592 nm, STED 660 nm, STED 775 nm

Ludin Cube and Box with Active CO2 gas regulation


Location: O-513
Contact Person: Ilya Grigoriev / Eugene Katrukha