Deltavision OMXV4 BLAZE 3D SIM & TIRF

Primary tasks:

  • Ring-TIRF
  • 3D SIM
  • FRAP
  • DIC

Excitation lasers:
405 nm Vortran Stradus (100 mw)
445 nm Omicron LuxX (100 mw)
488 nm Coherent (100 mw)
515 nm Coherent (100 mw)
568 nm Coherent (100 mw)
640 nm Vortran Stradus (110 mw)

LED excitation:
Lumencor spectra X

Objectives:
60x/1.49 TIRF (Olympus APO TIRFM, WD=0.1)
60x/1.42 SIM (Olympus U-PLAN APO, WD=0.15)

Dichroics:
BGR: DAPI/FITC/Alexa568/Cy5
CYR: CFP/YFP/mCherry/Cy5

Camera: 4x PCO sCMOS (~50% QY)

Environmental stage: OKO-lab (temperature, humidity and CO2 control)

Focus stabilization: Ultimate focus (IR laser)

Software: OMX software 

Installation: 2024

 

Location: O-515
Contact Person: Ernest van der Wee