Super-Resolution STORM/PALM custom made system

Primary tasks:
3D Single Molecule Localization Microscopy using Adaptive Optics

Microscope: Nikon Eclipse Ti with Perfect Focus System 3

Objectives:

Nikon Apo TIRF 100x/1.49 NA oil 
Nikon PLAN FLUOR 10x/0.3 NA air

Camera: 2 x Hamamatsu Flash 4.0v2

Lasers:
Omicron BrixX 638 nm (500mW multi-mode)
Coherent Obis 561 nm (150mW)
Omicron LuxX 491 nm (200mW)
Omicron LuxX 405 nm (60mW)

Stage: PILine Ultrasonic Linear Piezomotor PInano Z Piezo

Optical table: TMC High Performance Lab Table

Software: Micromanager (www.micro-manager.com)

Adaptive optics module: Imagine Optic MiCAO 3D-SR

Installation: 2013

Location: Z-512
Contact Person: Eugine Katrukha / Lukas Kapitein