Upright fluorescence microscope Nikon Eclipse 80i

Helma van Riel and master student Kim Boonekamp are checking microtubule binding of kinesin Kif21A mutants

Primary tasks: Imaging of fixed samples

Microscope: Nikon Eclipse 80i

Objective:

• Plan Apo VC 100x N.A. 1.40 oil
• Plan Apo VC 60x N.A. 1.40 oil
• Plan Fluor 40x N.A. 1.30 oil
• Plan Fluor 20x N.A. 0.75 oil
• Plan Fluor 10x N.A. 0.30
• Plan Fluor 4x N.A. 0.13

Camera: Photometrics CoolSNAP MYO CCD

Filters:

• Chroma ET-DAPI (49000)
• Chroma ET-BFP2 (49021)
• Chroma ET-GFP (49002)
• Chroma ET-mCherry (49008)
• Chroma ET-GFP/mCherry (59022)
• Chroma ET-Cy5 (49006)

Software: Nikon NIS Br

Installation: 2011

Location: O-512
Contact Person: Ilya Grigoriev