Category: Cell Biology

Super-Resolution STORM/PALM custom made system

Primary tasks:
3D Single Molecule Localization Microscopy using Adaptive Optics

Microscope: Nikon Eclipse Ti with Perfect Focus System 3

Objectives:

Nikon Apo TIRF 100x/1.49 NA oil 
Nikon PLAN FLUOR 10x/0.3 NA air

Camera: 2 x Hamamatsu Flash 4.0v2

Lasers:
Omicron … Read more

Posted in Equipment

TIRF Nikon Ti – Roper iLas-2 FRAP/Photoablation

TIRF 3 Nikon-Roper ILAS-2 PhotoAblation
Kai Jiang is studying the microtubule minus end proteins in the cells

Primary tasks:
TIRF microscopy, Single Molecule Imaging, FRAP, Photo-Activation, Photo-Ablation (532 nm)

Microscope: Nikon Eclipse Ti with Perfect Focus System

Objective: Apo TIRF 100x / 1.49, oil, WD=0.09-0.16 … Read more

Posted in Equipment

Super resolution and confocal microscope Leica TCS SP8 STED3X SMD FLIM

Confocal Leica SP8 FLIM STED3X super-resolution
Joanna Lipka is studying the microtubules in primary hippocampal neurons  

Primary tasks:

  • STED super-resolution imaging
  • Confocal live cell microscopy
  • FLIM
  • FCS

Microscope
Leica DMI 6000 CS AFC with Adaptive Focus Control
Confocal module with filter free spectral detection, 2 … Read more

Posted in Equipment

Confocal Laser Scanning microscope Zeiss LSM700 (2)

Confocal Zeiss LSM 700-2

Maud Martin is studying microtubules in endothelial cells

 

Primary tasks:

  • Confocal microscopy
  • 3D imaging
  • FRAP

Microscope: Axio Observer Z1 (inverted) (Zeiss, Germany)

Stage: motorized (Märzhäuser, Germany)

Objectives:

    EC Plan-Neofluar 10x/0.3  WD=5.2mm, Air (Zeiss, Germany)
    Plan-Apochromat … Read more

Posted in Equipment

Zeiss RoadShow

2014.09.25
Zeiss RoadShow

2014.09.25-Zeiss-RoadShow

 

MiCafee / UMCRead more

Posted in Activities, BIC

Testing Zeiss Imager.Z2 upright fluorescence microscope

2014.09.17
Testing Zeiss Imager.Z2 upright fluorescence microscope

2014.09.17-Testing-Zeiss-Imager.Z2--1

2014.09.17-Testing-Zeiss-Imager.Z2--2

 

Cell Biology Utrecht University, Room O-513Read more

Posted in Activities, BIC

Testing Nikon Ti-LAPP Modular Illumination System

2014.06.26
Testing Nikon Ti-LAPP Modular Illumination System

2014.06.26-Testing-Nikon-Ti-LAPP

 

Cell Biology Utrecht University, Room O-513Read more

Posted in Activities, BIC

Testing Nikon Ni-E upright fluorescence microscope

2014.06.18
Testing Nikon Ni-E upright fluorescence microscope

2014.06.18-Testing-Nikon-Ni-E

 

Cell Biology Utrecht University, Room O-513Read more

Posted in Activities, BIC