Confocal Spinning Disc 4 Nikon Ti2 – Yokogawa W1 – Gataca iLas FRAP/Photoablation system

Primary tasks:

Confocal live cell imaging
3D imaging
FRAP
Optogenetics

Microscope: Eclipse Ti2-E with PFS (Nikon, Japan)

Stage: MS-2000-XYZ with Piezo Top Plate (ASI, USA)

Piezo-Z: MS-2000-XYZ with Piezo Top Plate (ASI, USA)

Confocal Spinning Disc Unit: CSU-W1-T1 (Yokogawa, Japan)

FRAP: ILas (Gataca Systems, France)

Objectives:
     Plan FL LWD 20x / 0.70, WD=2.3-1.3, MRH08250, (Nikon, Japan), 3.10 pix/µm or 323 nm/pix
     Plan Apo λD 20x / 0.80, WD=0.80, MRD70270, (Nikon, Japan), 3.06 pix/µm or 327 nm/pix
     Plan Fluor 40x / 1.30, WD=0.24, oil, MRH01401 (Nikon, Japan), 5.96 pix/µm or 168 nm/pix
     Plan Apo λD 60x / 1.42, WD 0.15, oil, MRD71670 (Nikon, Japan), 8.83 pix/µm or 113 nm/pix
     Plan Apo λD 100x / 1.45, WD 0.13, oil, MRD71970 (Nikon, Japan), 14.63 pix/µm or 68 nm/pix
     Plan Apo VC 60x / 1.20, WD 0.31-0.28, water, MRD07602 (Nikon, Japan), 9.01 pix/µm or 111 nm/pix

Laser lines:
Vortran Stradus 405 (100 mW) (Vortran, USA)
    Vortran Stradus 445 (80 mW) (Vortran, USA)
    Vortran Stradus 488 (150 mW) (Vortran, USA)
    Coherent OBIS 561 nm (150 mW) (Coherent, USA)
    Vortran Stradus 642 (160 mW) (Vortran, USA)

Filters:
ET460/50m (DAPI), ET480/30m (CFP), ET525/50m (GFP), ET595/44m, ET605/70m (TRITC),
ET630/75m (mCherry), ET700/75m (Cy5), ET708/75m (Chroma, USA)

Beam Splitter: OptoSplit II two-channels for simultaneous colocalization (Cairn Research, UK) with filters
ET525/50m (GFP), ET630/75m (mCherry) and T585lpxr dichroic (Chroma, USA) or
ET525/50m (GFP), ET605/70m (mCherry) and T565lpxr dichroic (Chroma, USA)

Detectors: Prime BSI sCMOS camera (2048×2048 pixels) (Teledyne Photometrics, USA)

Temperature control and CO2: STXG-PLAMX-SETZ21L (TokaiHit, Japan)

Software: MetaMorph 7.10 (Molecular Devices, USA)

Installation: 2022

Location: Z-515
Contact Person: Ilya Grigoriev