High NA single objective lightsheet (Snouty) & STORM setup for Spectral Unmixing

Primary tasks:

• Ultrafast, high NA, single objective lightsheet volumetric microscopy (SOLM)
• (3D) far-red multi-color single-molecule localization microscopy (STORM)

Microscope base: Nikon Eclipse Ti with Perfect Focus System

Stage: Applied Scientific Instrumentation MS-2000

Heating/CO2: Tokai Hit INUBG2E-ZILCS Stage Top Incubator

Optical table: TMC High-Capacity Lab Table

Beamsplitter: Optosplit 3

Objectives:
SOLM
Primary:   Nikon SR HP Apo 100x/1.35 NA silicone 
Secondary:  Nikon Plan Apo Lambda D 40x/0.95 NA air 
Tertiary:  Calico/ASI AMS-AGY v.1.1 1.0 NA solid immersion
STORM
Nikon SR HP Apo 100x/1.35 NA silicone 
Nikon Apo TIRF 100x/1.49 NA oil

Camera:
SOLM
Andor Zyla 4.2 sCMOS (air cooled) 
STORM
Andor iXon EMCCD

Lasers:
SOLM 
Cobolt Calypso 491 nm  
Cobolt Jive 561 nm  
PTI IQ1C35 640 nm
STORM
PTI IQ2C 405 nm 
Omicron BrixX 638 nm (500mW multi-mode)

SOLM scanner: Scandreas lensless scanner (home-built)

3D STORM: Nikon N-STORM

Software:
SOLM: Custom made Python controller software
STORM: Micromanager

Installation: 2021 

Location: Z-514
Contact Person: Ernest van der Wee / Eugene Katrukha