Confocal Spinning Disc 2 Nikon Ti – Yokogawa X1 – Gataca iLAS FRAP/Photoablation system

Spinning Disc 2 system
Marina Mikhaylova and Philipp Schätzle are studying the synaptic targeting of the protein in dissociated neurons.

Primary tasks:

  • Confocal live cell imaging
  • 3D imaging
  • FRAP
  • Optogenetics
  • Photo-Ablation

Microscope: Eclipse Ti-E with PFS (Nikon, Japan)

Stage: MS-2000-XYZ with Piezo Top Plate (ASI, USA)

Piezo-Z: MS-2000-XYZ with Piezo Top Plate (ASI, USA)

Confocal Spinning Disc Unit: CSU-X1-A1 (Yokogawa, Japan)

FRAP: ILas (Roper Scientific France/ PICT-IBiSA, Institut Curie; now Gataca Systems, France)

PhotoAblation: 355 nm Q-switched pulsed laser (Teem Photonics, France)

Objectives:

    Plan Apo 20x / 0.75, multi-immersion, WD=0.35 (Nikon, Japan)
    Plan Fluor 40x / 1.30, oil, WD=0.24 (Nikon, Japan), 6.3 pix/µm or 159 nm/pix
    Plan Apo VC 60x / 1.40, oil, WD=0.15 (Nikon, Japan), 9.4 pix/µm or 106 nm/pix
    Plan Apo VC 100x / 1.40, oil, WD=0.16 (Nikon, Japan), 15.59 pix/µm or 64 nm/pix
    S Fluor 100x / 0.5-1.3, oil, WD=0.2 (Nikon, Japan), 15.59 pix/µm or 64 nm/pix (for 355 nm)

Light source (laser lines):

    Vortran Stradus 405 nm (100 mW) (Vortran, USA)
    Vortran Stradus 488 nm (150 mW) (Vortran, USA)
    Coherent OBIS 561 nm (100 mW) (Coherent, USA)
    Vortran Stradus 642 nm (150 mW) (Vortran, USA)

Light source (LEDs): CoolLED pE-300 White (CoolLED, UK)

Filters: ET460/50m (DAPI), ET525/50m (GFP), ET630/75m (mCherry), ET700/75m (Cy5), (Chroma, USA)

Beam Splitter: OptoSplit II two-channels for simultaneous colocalization (Cairn Research, UK) with filters ET525/50m (GFP), ET630/75m (mCherry) and T585lpxr dichroic (Chroma, USA)

Detectors: Prime BSI sCMOS camera (2048×2048 pixels) (Teledyne Photometrics, USA)

Temperature control and CO2: STXG-PLAMX-SETZ21L (TokaiHit, Japan)

Software: MetaMorph 7.10 (Molecular Devices, USA)

Installation: 2013

Location: O-524
Contact Person: Ilya Grigoriev