Spinning Disc 2 Nikon-Roper ILAS PhotoAblation

Spinning Disc 2 system
Marina Mikhaylova and Philipp Schätzle are studying the synaptic targeting of the protein in dissociated neurons.

Primary tasks:
Confocal Spinning Disc microscopy, 3D imaging

Microscope: Nikon Eclipse Ti with Perfect Focus System (3rd generation)

Confocal Head: Yokogawa CSU-X1-A1

Plan Apo VC 100x N.A. 1.40 oil
Plan Apo 60x N.A. 1.40 oil
Plan Fluor 40x N.A. 1.30 oil
Plan Apo 20x N.A. 0.75 oil
Plan Apo 10x N.A. 0.45 dry

Beamsplitter: DV2 (Two-channel, simultaneous-imaging system)

Camera: Photometrics Evolve 512 EMCCD

Vortran Stradus 405 nm (100 mW)
Cobolt Calypso 491 nm (100 mW)
Cobolt Jive 561 nm (100 mW)
Vortran Stradus 642 nm (110 mW)

ET-BFP2 (49021), ET-GFP (49002), ET-mCherry (49008), ET-Cy5 (49006),
ET-GFPmCherry (59022) – all Chroma

Stage: ASI motorized stage MS-2000-XYZ (linear encoder option) with Piezo Top Plate

Heating: Tokai Hit INUBG2E-ZILCS Stage Top Incubator
Software: MetaMorph 7.7

Resolution: 67 nm in 1 pixel (100x objective)

Location: O-524
Contact Person: Ilya Grigoriev