Super-resolution STORM/PALM home made system (N2)
Primary tasks:
3D Single Molecule Localization Microscopy using Adaptive Optics
Microscope:
Nikon Eclipse Ti with Perfect Focus System 3
Objectives:
Nikon Apo TIRF 100x N.A. 1.49 oil
Beamsplitter:
Optosplit 3 LS (allows simultaneous three-color and ratiometric imaging with large field of view cameras)
Camera:
2 x Hamamatsu Flash 4.0v2
Lasers:
Omicron BrixX 638 nm (500mW multi-mode)
Coherent Obis 561 nm (150mW)
Omicron LuxX 491 nm (200mW)
Omicron LuxX 405 nm (60mW)
Stage:
PILine Ultrasonic Linear Piezomotor PInano Z Piezo
Optical table:
TMC High Performance Lab Table
Software:
Micromanager (www.micro-manager.com)
Other equipment:
Imagine Optic MiCAO 3D-SR
Location: Z-512
Contact Person: Lukas Kapitein