Super-Resolution STORM/PALM home made system -2

Super-resolution STORM/PALM home made system (N2)

Primary tasks:
3D Single Molecule Localization Microscopy using Adaptive Optics

Microscope:
Nikon Eclipse Ti with Perfect Focus System 3

Objectives:
Nikon Apo TIRF 100x N.A. 1.49 oil

Beamsplitter:
Optosplit 3 LS (allows simultaneous three-color and ratiometric imaging with large field of view cameras)

Camera:
2 x Hamamatsu Flash 4.0v2

Lasers:
Omicron BrixX 638 nm (500mW multi-mode)
Coherent Obis 561 nm (150mW)
Omicron LuxX 491 nm (200mW)
Omicron LuxX 405 nm (60mW)

Stage:
PILine Ultrasonic Linear Piezomotor PInano Z Piezo

Optical table:
TMC High Performance Lab Table

Software:
Micromanager (www.micro-manager.com)

Other equipment:
Imagine Optic MiCAO 3D-SR

Location: Z-512
Contact Person: Lukas Kapitein