Upright FL microscope Nikon Eclipse 80i

Upright fluorescence microscope Nikon Eclipse 80i
Helma van Riel and master student Kim Boonekamp are checking microtubule binding of kinesin Kif21A mutants


Primary tasks:
Imaging of fixed samples

Microscope: Nikon Eclipse 80i

Objective:

  • Plan Apo VC 100x N.A. 1.40 oil
  • Plan Apo VC 60x N.A. 1.40 oil
  • Plan Fluor 40x N.A. 1.30 oil
  • Plan Fluor 20x N.A. 0.75 oil
  • Plan Fluor 10x N.A. 0.30
  • Plan Fluor 4x N.A. 0.13

Camera: Photometrics CoolSNAP HQ2 CCD

Filters:

  • Chroma ET-DAPI (49000)
  • Chroma ET-BFP2 (49021)
  • Chroma ET-GFP (49002)
  • Chroma ET-mCherry (49008)
  • Chroma ET-GFP/mCherry (59022)
  • Chroma ET-Cy5 (49006)

Software: Nikon NIS Br

 

Location: O-512
Contact Person: Ilya Grigoriev / Eugene Katrukha