Confocal Leica SP8 FLIM STED3X super-resolution

Super Resolution and confocal microscope Leica TCS SP8 STED3X SMD FLIM

Confocal Leica SP8 FLIM STED3X super-resolution
Joanna Lipka is studying the microtubules in primary hippocampal neurons  

Primary tasks:

  • STED super-resolution imaging
  • Confocal live cell microscopy
  • FLIM
  • FCS

Microscope
Leica DMI 6000 CS AFC with Adaptive Focus Control
Confocal module with filter free spectral detection, 2 PMT and 3 HyD detectors
Gated and 3D STED
Single molecule TCSPC system PicoHarp 300

Objectives
20x (dry) HC PL FLUOTAR 20x/ NA 0.4 CORR (working distance 7mm)
20x (oil) HC PL APO 20x/NA 0.75 IMM CORR CS2
40x (oil) HC PL APO 40x/NA 1.30 OIL CS2
63x (oil) HC PL APO 63x/NA 1.40 OIL CS2
63x (water) HC PL APO 63x/1.20 W CORR CS2
86x (water) HC PL APO 86x/NA 1.20 W motCORR STED
93x (glycerol) HC PL APO 93x/1.30 GLYC motCORR STED
100x (oil) HC PL APO 100x/1.40 STED

Laser lines
405 nm DMOD Flexible
440 pulsed FLIM
White Laser (470 670 nm)
Argon laser
STED 592 nm, STED 660 nm, STED 775 nm

37oC:
Ludin Cube and Box with Active CO2 gas regulation

 

Location: O-513
Contact Person: Ilya Grigoriev / Eugene Katrukha