TIRF Nikon Ti – Roper iLas-2 FRAP/Photoablation

Kai Jiang is studying the microtubule minus end proteins in the cells

Primary tasks:
TIRF microscopy, Single Molecule Imaging, FRAP, Photo-Activation, Photo-Ablation (532 nm)

Microscope: Nikon Eclipse Ti with Perfect Focus System

Objective: Apo TIRF 100x / 1.49, oil, WD=0.09-0.16 (Nikon, Japan)

Beamsplitter: Optosplit III (allows two or three color simultaneous imaging)

Camera:

Evolve Delta 512 EMCCD (512×512 pixels) (Teledyne Photometrics, USA)
CoolSNAP Myo CCD (1940×1460 pixels) (Teledyne Photometrics, USA)

Lasers:

Vortran Stradus 405 nm (100 mW) (Vortran, USA)
Vortran Stradus 445 nm (80 mW) (Vortran, USA)
Vortran Stradus 488 nm (150 mW) (Vortran, USA)
Coherent OBIS 561 nm (100 mW) (Coherent, USA)
Vortran Stradus 642 nm (110 mW) (Vortran, USA)
Teem Photonics 532 nm (for photoablation) (Teem Photonics, France)

FRAP/TIRF: Ilas-3 system (Roper Scientific France/ PICT-IBiSA, Institut Curie; now Gataca Systems, France)

Filters:

ET-BFP2 (49021), ET-GFP (49002), ET-mCherry (49008), ET-GFPmCherry (59022) – all Chroma

Stage: ASI motorized stage MS-2000-XY System for Inverted Microscope Nikon Te/Ti 2000

Filter Wheels: Sutter LB10-3 (excitation filter wheel with smart shutter; emission filter wheel; smart shutter for transmission light)

Heating: Tokai Hit STXG-PLAMX-SETZ21L Stage Top Incubator

Software: MetaMorph 7.10

Resolution: 64 nm in 1 pixel (Evolve camera) / 45.4 nm in 1 pixel (CoolSNAP Myo camera)

Installation: 2015

Location: O-524
Contact Person: Ilya Grigoriev