Alfredo Rates Soriano making cells running in circles
Primary tasks:
• Optogenetics
• Long-term imaging
• DIC
• Phase-Contrast
Microscope: Nikon Ti2
Fluorescence Filters: ET-DAPI (49028), ET-Narrow Band EGFP (49020), ET- 514nm for EYFP (49905), ET-mCherry (49008), ET-CY5 (49006) – all Chroma Alexa Fluor 700 – Photonics Solutions
Objectives:
CFI Plan Apo λD 10x/0.45 air DIC(WD = 4 mm)
CFI Plan Apo λD 20x/0.8 air DIC(WD = 0.8 mm)
CFI Plan Fluor 20x/0.75 multi imm. DIC(WD = 0.51-0.33 mm)
CFI Plan Fluor 40x/1.3 oil DIC/PC (WD = 0.24 mm)
CFI Plan Apo λD 60x/1.42 oil DIC/PC (WD = 0.15 mm)
Camera:
Andor SONA sCMOS (back-illuminated, 2048×2046, 6.5 µm pixel size)
Moment MONO sCMOS (for phase contrast, 3200×2200, 4.5 µm pixel size)
Digital Mirror Device:
Andor Mosaic3 Digital Micromirror Device (800×600) for patterned illumination with 400, 435, 470, 500, 550, 580, 635 and 740 nm LEDs.
Temperature control and CO2: Tokai Hit chamber
Software: MicroManager
Installation: 2024
Location: O-515
Contact Person: Ilya Grigoriev/ Ernest van der Wee