Wide-field Nikon Ti “PEX”-scope

Sam van Beuningen and Mariella Franker are studying the molecular motors using inducible PEX assay

Primary tasks:
• Long-term imaging
• DIC
• Phase-Contrast

Microscope: Nikon Ti

Fluorescence Filters: ET-EBFP2 (49021), ET-GFP (49002), ET-mCherry (49008), ET-Qdot 625 – all Chroma

Objectives:

    ADL 10x/0.25 Phase Contrast (WD = 6.2 mm)
    Plan Apochromat 20x/0.75 Phase Contrast  (WD = 1.00 mm)
    Plan Apo 40x/1.0 Oil Phase Contrast (WD = 0.16 mm)
    Plan Fluor 40x/1.30 Oil DIC (WD = 0.20 mm)
    Plan Apo 60x/1.4 Oil Phase Contrast (WD = 0.3 mm)

Camera: pco.edge 4.2 sCMOS

Digital Mirror Device: Mightex Polygon Digital Mirror Device for patterned illumination with 405, 470, 625 and 740 nm LEDs.

Software: MicroManager

Installation: 2012

Location: Z-515
Contact Person: Ilya Grigoriev/ Eugene Katrukha