Re-scan Confocal microscope RCM1

Primary tasks:

• Laser scanning confocal (automated super resolution)
• Widefield fluorescence microscopy
• 3D imaging

Microscope: Nikon Ti-U

Stage: TI-S-E Motorized stage

Objectives:
     Plan fluor 10x / 0.30 (Nikon, Japan) 867 nm/pix
     Plan fluor 20x / 0.50 (Nikon, Japan) 433 nm/pix
     Plan apo VC 60x / 1.40, oil (Nikon, Japan) 144 nm/pix
     Plan Apo VC 100x / 1.40, oil (Nikon, Japan) 87 nm/pix

Light source (laser lines):
    iChrome CLE 405 nm / 19.3 mW (Toptica, Germany)
    iChrome CLE 488 nm / 19.1 mW (Toptica, Germany)
    iChrome CLE 561 nm / 23.0 mW (Toptica, Germany)
    iChrome CLE 640 nm / 21 mW (Toptica, Germany)

Light source (LEDs): CoolLED pE-300 White MB (CoolLED, UK)

Filters:
   “530” GFP-L [EX 460-500 / DM 505 / BA 510]
   “600” TxRed [EX 540-580 / DM 595 / BA 600-660]
   “DIC” Nikon TI-A

Emission filter: Infinity optics wheel with ZET405/488/561/640 filters

Detectors: Flash 4.0 v3 (2048×2048 pixels) (Hamamatsu, Japan)

Software: MicroManager 

Installation: 2021

Location: O-518
Contact Person: Ruben Schmidt