Zeiss Apotome 3 optical sectioning microscope

Primary tasks:

• 3D imaging

Microscope: Axio Imager Z2 (upright)

Stage: motorized (Märzhäuser, Germany)

Objectives:
     Plan-Neofluar 10x/0.3 WD=5.2mm, Air
     Plan-Apochromat 20x/0.8 WD=0.55mm, Air
     C-Apochromat 40x/1.20 WD=0.28mm, Water Immersion
     Plan-Apochromat 40x/1.3 WD=0.21mm, Oil Immersion (Zeiss, Germany)
     Plan-Apochromat 63x/1.4  WD=0.19mm, Oil Immersion (Zeiss, Germany)

Light sources: LED Colibri 7: 385 nm, 430 nm, 475 nm, 511 nm, 555 nm, 590 nm, 630 nm

Filters:
     38 HE eGFP, Zeiss (450-490 / 495 / 500-550)
     43 HE DsRed, Zeiss (538-562 / 570 / 570-640)
     50 Cy5, Zeiss (625-655 / 660 / 665-715)
     70 HE Alexa Fluor 430, Zeiss (400-460 / 500 / 500-600)
     96 HE BFP, Zeiss (370-410 / 420 / 430-470)
     118 HE YFP, Zeiss (500-515 / 525 / 535-575)
     Chlorofyl LP, Zeiss (465-495 / 505 / LP600)
     00 Propidium Iodide, Zeiss (530-585 / 600 / LP615) 

Detectors: Prime BSI express cCMOS (Photometrics)

Software: ZEN Blue v3.7

Installation: 2023

Location: Z-316
Contact Person: Daan Weits / Ilya Grigoriev