Zeiss Lattice Lightsheet LL7 system
Malina Iwanski is using LL7 system.
Primary task: Lattice lightsheet imaging of single cells
Excitation lens: 13.3x / 0.44
Observation lens: 44.83x/1
Light Sheet: 15×550, 15×650
30×700, 300×1000
100×1400, 100×1800
Lasers: 488, 561, 640
Cameras: Two … Read more
Confocal Laser Scanning microscope Zeiss LSM880 Fast AiryScan
Boris Shneyer and Sanne Remmelzwaal are discussing microtubule nucleation in differentiated primary muscle cells.
Primary tasks:
- Confocal microscopy
- 3D imaging
- SuperResolution
Microscope: inverted Axio Observer 7 SP with Definite Focus 2 (Zeiss, Germany)
Stage: scanning stage 130×100 STEP Set LSM … Read more
Digital inverted fluorescent microscope AMG EVOS
Qingyang Liu and Sanne Remmelzwaal are checking the HeLa cells transfected with wild-type Rab8 protein.
Primary tasks: Cell Culture Observation
Microscope: AMG EVOS
Objective:
- LPlan PH2 4x/0.13 phase-contrast
- Plan Fluor 10x/0.3
- Plan Fluor 20x/0.5
- LPlan PH2 20x/0.40 phase-contrast
Epi-fluorescence: GFP, … Read more
Upright fluorescence microscope Nikon Ni-E
Jingchao Wu is studying
Primary tasks: Imaging of fixed samples
Microscope: Nikon Eclipse Ni-E
Objective:
- Plan Apo Lambda 100x N.A. 1.45 oil
- Plan Apo Lambda 60x N.A. 1.40 oil
- Plan Fluor 40x N.A. 1.30 oil
- Plan Fluor 20x N.A. 0.75
…
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Super-Resolution STORM/PALM custom made system
Primary tasks:
3D Single Molecule Localization Microscopy using Adaptive Optics
Microscope: Nikon Eclipse Ti with Perfect Focus System 3
Objectives:
Nikon Apo TIRF 100x/1.49 NA oil
Nikon PLAN FLUOR 10x/0.3 NA air
Camera: 2 x Hamamatsu Flash 4.0v2
Lasers:
Omicron … Read more
TIRF Nikon Ti – Roper iLas-2 FRAP/Photoablation
Kai Jiang is studying the microtubule minus end proteins in the cells
Primary tasks:
TIRF microscopy, Single Molecule Imaging, FRAP, Photo-Activation, Photo-Ablation (532 nm)
Microscope: Nikon Eclipse Ti with Perfect Focus System
Objective: Apo TIRF 100x / 1.49, oil, WD=0.09-0.16 … Read more
Super resolution and confocal microscope Leica TCS SP8 STED3X SMD FLIM
Joanna Lipka is studying the microtubules in primary hippocampal neurons
Primary tasks:
- STED super-resolution imaging
- Confocal live cell microscopy
- FLIM
- FCS
Microscope
Leica DMI 6000 CS AFC with Adaptive Focus Control
Confocal module with filter free spectral detection, 2 … Read more
Confocal Laser Scanning microscope Zeiss LSM700 (2)
Maud Martin is studying microtubules in endothelial cells
Primary tasks:
- Confocal microscopy
- 3D imaging
- FRAP
Microscope: Axio Observer Z1 (inverted) (Zeiss, Germany)
Stage: motorized (Märzhäuser, Germany)
Objectives:
EC Plan-Neofluar 10x/0.3 WD=5.2mm, Air (Zeiss, Germany)
Plan-Apochromat … Read more