Cell Biology, Neurobiology and Biophysics

Neuro retreat

Each spring we go to Fort aan de Klop (link: http://www.fortaandeklop.com/) to discuss neuroscience topics that are interesting, but normally outside our scope, in an informal setting. The aim of the retreat is to motivate and inspire, to broaden … Read more

Digital inverted fluorescent microscope AMG EVOS

Digital inverted fluorescent microscope AMG EVOS
Qingyang Liu and Sanne Remmelzwaal are checking the HeLa cells transfected with wild-type Rab8 protein.

Primary tasks: Cell Culture Observation

Microscope: AMG EVOS

Objective:

  • LPlan PH2 4x/0.13 phase-contrast
  • Plan Fluor 10x/0.3
  • Plan Fluor 20x/0.5
  • LPlan PH2 20x/0.40 phase-contrast

Epi-fluorescence: GFP, … Read more

Upright fluorescence microscope Nikon Ni-E

Upright FL microscope Nikon Eclipse Ni
Jingchao Wu is studying

Primary tasks: Imaging of fixed samples

Microscope: Nikon Eclipse Ni-E

Objective:

  • Plan Apo Lambda 100x N.A. 1.45 oil
  • Plan Apo Lambda 60x N.A. 1.40 oil
  • Plan Fluor 40x N.A. 1.30 oil
  • Plan Fluor 20x N.A. 0.75
Read more

Super-Resolution STORM/PALM custom made system

Primary tasks:
3D Single Molecule Localization Microscopy using Adaptive Optics

Microscope: Nikon Eclipse Ti with Perfect Focus System 3

Objectives:

Nikon Apo TIRF 100x/1.49 NA oil 
Nikon PLAN FLUOR 10x/0.3 NA air

Camera: 2 x Hamamatsu Flash 4.0v2

Lasers:
Omicron … Read more

TIRF Nikon Ti – Roper iLas-2 FRAP/Photoablation

TIRF 3 Nikon-Roper ILAS-2 PhotoAblation
Kai Jiang is studying the microtubule minus end proteins in the cells

Primary tasks:
TIRF microscopy, Single Molecule Imaging, FRAP, Photo-Activation, Photo-Ablation (532 nm)

Microscope: Nikon Eclipse Ti with Perfect Focus System

Objective: Apo TIRF 100x / 1.49, oil, WD=0.09-0.16 … Read more

Confocal Laser Scanning microscope Zeiss LSM700 (2)

Confocal Zeiss LSM 700-2

Maud Martin is studying microtubules in endothelial cells

 

Primary tasks:

  • Confocal microscopy
  • 3D imaging
  • FRAP

Microscope: Axio Observer Z1 (inverted) (Zeiss, Germany)

Stage: motorized (Märzhäuser, Germany)

Objectives:

    EC Plan-Neofluar 10x/0.3  WD=5.2mm, Air (Zeiss, Germany)
    Plan-Apochromat … Read more