Confocal Laser Scanning microscope Zeiss LSM900

Primary tasks:

• Confocal microscopy
• 3D imaging
• FRAP

Microscope: upright Axio Imager Z2 (Zeiss, Germany)

Stage: Scanning stage 130×85 STEP Set LSM (Märzhäuser, Germany)

Objectives:
     EC Plan-Neofluar 10x/0.3  WD=5.2mm, Air (Zeiss, Germany)
     Plan-Apochromat 20x/0.8 WD=0.55mm, Air (Zeiss, Germany)
     Plan-Apochromat 40x/1.0 WD=2.5mm, Water-dipping
     Plan-Apochromat 40x/1.3 WD=0.21mm, Oil Immersion (Zeiss, Germany)
     Plan-Apochromat 63x/1.4  WD=0.19mm, Oil Immersion (Zeiss, Germany)

Light source (laser lines): 405nm, 488nm, 561nm and 640 nm

Light source (LEDs): Colibri 5 (385 nm, 475 nm, 555 nm, 630 nm) (Zeiss, Germany)

Detectors: two multialkali PMT (MA-PMT) (Scan module LSM900) (Zeiss, Germany)

 Software: ZEN Blue v3.7 (Zeiss, Germany)

Installation: 2023

Location: O-525
Contact Person: Ruben Schmidt/ Ilya Grigoriev