Cell Biology, Neurobiology and Biophysics

Confocal Laser Scanning microscope Zeiss LSM910 AiryScan 2

              Zoé Ducarne imaging expanded tissue

 

Primary tasks:

  • Confocal microscopy
  • 3D imaging
  • Super-resolution imaging

Microscope: Axio Observer 7 with Definite Focus 3

Stage: motorized, Märzhäuser

Piezo-Z: yes, stage

Objectives:
    Plan-Neofluar 5x/0.16 WD=18.5mm, Air
    Plan-Apochromat 20x/0.8 WD=0.55mm, Air
    C-Apochromat 40x/1.20 WD=0.28mm, Water Immersion

Light sources:
    Laser lines: 405 nm (5 mW), 488 nm (10mW), 561 nm (10mW), 640 nm (5 mW)
    LED Viluma 5: 385 nm, 469 nm, 555 nm, 631 nm

Detectors:
    Camera
    Axiocam 305 mono (D), 5.07 megapixel, 2464×2056,
    SONY IMX264 CMOS sensor, 8/12 bit, 65% QE
    MA-PMT: two
    GaAsP: one
    AiryScan: one

DIC: yes

Temperature control and CO2: no

Software: ZEN Blue v3.13

Installation: 2026

 

Location: O-525
Contact Person: Ilya Grigoriev