Cell Biology, Neurobiology and Biophysics

Olympus FVMPE-RS Two-photon upright microscope with gantry frame

Ting-Feng Lin looking for the right neurons

 

Lasers:

  • Insight X3 (680-1300nm, ~2.5W)
  • MaiTai HPDS (690-1040nm, ~2W)

Epifluorescence excitation:

  • Olympus U-HGLGPS light source

Epifluorescence cubes:

  • U-FUW (Ex340-390, DM410, Em420+)
  • U-FBN (Ex470-495, DM505, Em510+)
  • U-FGWA (Ex530-550, DM570, 575-625)

Objective:

  • 25x/1.05 Water (Olympus XLPlan N, WD=2 mm, 0-0.23 mm cover glass thickness)

Dichroic Cubes:

  • FV30-SDMOPT (SDM565R)
  • FV30-SDM570 (SDM570)

Filter Cubes:

  • FV30-FOPT (DM495, EM502-547, EM415-485)
  • FV30-FRCY5 (DM650, EM575-645, EM660-750)

Detectors: 2x GaAsP, 2x PMT

Scanners:

  • Galvanometric scanner (for standard imaging)
  • Resonant scanner (for high speed imaging)
  • SIM scanner (for stimulation/optogenetics)

Software: Fluoview

 

Installation: 2026

 

Location: O-515
Contact Person: Ernest van der Wee