Confocal Laser Scanning microscope Zeiss LSM980 AiryScan 2

Primary tasks:

• Confocal microscopy
• 3D imaging
• Super-resolution imaging

Microscope: Axio Observer 7 SP with Definite Focus 3

Stage: motorized, Märzhäuser

Piezo-Z: yes, stage

Objectives:
    Plan-Neofluar 5x/0.16 WD=18.5mm, Air
    Plan-Apochromat 20x/0.8 WD=0.55mm, Air
    C-Apochromat 40x/1.20 WD=0.28mm, Water Immersion
    C-Apochromat 63x/1.20 WD=0.28mm, Water Immersion
    Alpha Plan-APO 100x/1,46 WD=0.10mm, Oil Immersion

Light sources:
    Laser lines: 405 nm, 445 nm, 488 nm, 561 nm, 639 nm
    LED Colibri 5: 385 nm, 475 nm, 555 nm, 630 nm

Detectors:
    Camera
    Axiocam 305 mono R2, 5.07 megapixel, 2464×2056,
    SONY IMX264 CMOS sensor, 12 bit, 65% QE
    MA-PMT: two
    GaAsP: one
    AiryScan: one

Temperature control and CO2: yes, Tokai Hit

Software: ZEN Blue v3.9

Installation: 2023

Location: O-525
Contact Person: Ilya Grigoriev