Confocal Spinning Disc 1 Nikon Ti – Yokogawa X1 – Gataca iLAS FRAP/Photoablation system

Confocal Yokogawa Spinning Disc X1 Nikon Ti Roper iLas FRAPPhotoablation system
Microscopy facility manager Ilya Grigoriev, in front of Spinning Disc microscope

Primary tasks:

  • Confocal Spinning Disc microscopy
  • 3D imaging
  • FRAP
  • Photo-Activation
  • Photo-Ablation (355 nm)

Microscope: Eclipse Ti-E with PFS (Nikon, Japan)

Stage: MS-2000-XYZ with Piezo Top Plate (ASI, USA)

Piezo-Z: MS-2000-XYZ with Piezo Top Plate (ASI, USA)

Confocal Spinning Disc Unit: CSU-X1-A1 (Yokogawa, Japan)

FRAP: ILas (Roper Scientific France/ PICT-IBiSA, Institut Curie; now Gataca Systems, France)

PhotoAblation: 355 nm Q-switched pulsed laser (Teem Photonics, France)

Objectives:

    Plan Apo 20x / 0.75, multi-immersion, WD=0.35 (Nikon, Japan)
    Plan Fluor 40x / 1.30, oil, WD=0.24 (Nikon, Japan),  6.1 pix/µm or 164 nm/pix
    Plan Apo VC 60x / 1.40, oil, WD=0.15 (Nikon, Japan), 9.1 pix/µm or 109 nm/pix
    Apo TIRF 100x / 1.49, oil, WD=0.09-0.16 (Nikon, Japan), 15.1 pix/µm or 67 nm/pix
    S Fluor 100x / 0.5-1.3, oil, WD=0.2 (Nikon, Japan), 15.1 pix/µm or 67 nm/pix (for 355 nm)

Light source (laser lines):

     Vortran Stradus 405 nm (100 mW) (Vortran, USA)
     Vortran Stradus 445 nm (80 mW) (Vortran, USA)
     Vortran Stradus 488 nm (150 mW) (Vortran, USA)
     Cobolt Jive (561 nm, 110 mW) (Cobolt, Sweden)
     Vortran Stradus 642 nm (165 mW) (Vortran, USA)

Light source (LEDs): CoolLED pE-300 White (CoolLED, UK)

Filters: ET460/50m (DAPI), ET480/40m (CFP), ET525/50m (GFP), ET535/30m (YFP), ET630/75m (mCherry), ET700/75m (Cy5), 59017m (CFP/YFP), 59022m (GFP/mCherry); (Chroma, USA)

Beam Splitter: DV2 two-channels for simultaneous colocalization (Photometrics, USA) with filters

      ET525/50m (GFP), ET630/75m (mCherry) and T565lpxr dichroic (Chroma, USA) or 
      ET480/40m (CFP), ET535/30m (YFP) and T505lpxr dichroic (Chroma, USA)

Detectors: Evolve 512 EMCCD camera (512×512 pixels) (Teledyne Photometrics, USA)

DIC: Nikon, high-resolution short working distance

Temperature control and CO2: STXG-PLAMX-SETZ21L (TokaiHit, Japan)

Software: MetaMorph 7.10 (Molecular Devices, USA)

Installation: 2011

 

Location: Z-526
Contact Person: Ilya Grigoriev