Category: Equipment

Confocal Laser Scanning microscope Zeiss LSM900

 

Primary tasks:

  • Confocal microscopy
  • 3D imaging
  • FRAP

Microscope: upright Axio Imager Z2 (Zeiss, Germany)

Stage: Scanning stage 130×85 STEP Set LSM (Märzhäuser, Germany)

Objectives:
     EC Plan-Neofluar 10x/0.3  WD=5.2mm, Air (Zeiss, Germany)
     Plan-Apochromat 20x/0.8 WD=0.55mm, Air (Zeiss, Germany)… Read more

Posted in BIC, Equipment

Andor benchtop confocal BC43

 

Primary task: Long-term spinning disk confocal imaging of living cells and organoids.

Illumination: Brightfield and differential phase contrast transmitted white light, wide-field epifluorescence, Borealis™-illuminated multipoint confocal with 405/488/561/638 nm excitation lasers.

Objectives:
2x Plan UW Air … Read more

Posted in BIC, Equipment

Zeiss Lattice Lightsheet LL7 system


 Malina Iwanski is using LL7 system.

Primary task:  Lattice lightsheet imaging of single cells

Excitation lens:  13.3x / 0.44

Observation lens:  44.83x/1

Light Sheet:  15×550, 15×650

  30×700, 300×1000

  100×1400, 100×1800

Lasers:  488, 561, 640

Cameras:  Two … Read more

Posted in BIC, Equipment

Confocal Laser Scanning microscope Zeiss LSM880 Fast AiryScan


Boris Shneyer and Sanne Remmelzwaal are discussing microtubule nucleation in differentiated primary muscle cells.

Primary tasks:

  • Confocal microscopy
  • 3D imaging
  • SuperResolution

Microscope: inverted Axio Observer 7 SP with Definite Focus 2 (Zeiss, Germany)

Stage: scanning stage 130×100 STEP Set LSM … Read more

Posted in BIC, Equipment

Digital inverted fluorescent microscope AMG EVOS

Digital inverted fluorescent microscope AMG EVOS
Qingyang Liu and Sanne Remmelzwaal are checking the HeLa cells transfected with wild-type Rab8 protein.

Primary tasks: Cell Culture Observation

Microscope: AMG EVOS

Objective:

  • LPlan PH2 4x/0.13 phase-contrast
  • Plan Fluor 10x/0.3
  • Plan Fluor 20x/0.5
  • LPlan PH2 20x/0.40 phase-contrast

Epi-fluorescence: GFP, … Read more

Posted in Equipment

Upright fluorescence microscope Nikon Ni-E

Upright FL microscope Nikon Eclipse Ni
Jingchao Wu is studying

Primary tasks: Imaging of fixed samples

Microscope: Nikon Eclipse Ni-E

Objective:

  • Plan Apo Lambda 100x N.A. 1.45 oil
  • Plan Apo Lambda 60x N.A. 1.40 oil
  • Plan Fluor 40x N.A. 1.30 oil
  • Plan Fluor 20x N.A. 0.75
… Read more
Posted in Equipment

Super-Resolution STORM/PALM custom made system

Primary tasks:
3D Single Molecule Localization Microscopy using Adaptive Optics

Microscope: Nikon Eclipse Ti with Perfect Focus System 3

Objectives:

Nikon Apo TIRF 100x/1.49 NA oil 
Nikon PLAN FLUOR 10x/0.3 NA air

Camera: 2 x Hamamatsu Flash 4.0v2

Lasers:
Omicron … Read more

Posted in Equipment

TIRF Nikon Ti – Roper iLas-2 FRAP/Photoablation

TIRF 3 Nikon-Roper ILAS-2 PhotoAblation
Kai Jiang is studying the microtubule minus end proteins in the cells

Primary tasks:
TIRF microscopy, Single Molecule Imaging, FRAP, Photo-Activation, Photo-Ablation (532 nm)

Microscope: Nikon Eclipse Ti with Perfect Focus System

Objective: Apo TIRF 100x / 1.49, oil, WD=0.09-0.16 … Read more

Posted in Equipment