Primary tasks:
Microscope: upright Axio Imager Z2 (Zeiss, Germany)
Stage: Scanning stage 130×85 STEP Set LSM (Märzhäuser, Germany)
Objectives:
EC Plan-Neofluar 10x/0.3 WD=5.2mm, Air (Zeiss, Germany)
Plan-Apochromat 20x/0.8 WD=0.55mm, Air (Zeiss, Germany)… Read more
Primary task: Long-term spinning disk confocal imaging of living cells and organoids.
Illumination: Brightfield and differential phase contrast transmitted white light, wide-field epifluorescence, Borealis™-illuminated multipoint confocal with 405/488/561/638 nm excitation lasers.
Objectives:
2x Plan UW Air … Read more
Malina Iwanski is using LL7 system.
Primary task: Lattice lightsheet imaging of single cells
Excitation lens: 13.3x / 0.44
Observation lens: 44.83x/1
Light Sheet: 15×550, 15×650
30×700, 300×1000
100×1400, 100×1800
Lasers: 488, 561, 640
Cameras: Two … Read more
Boris Shneyer and Sanne Remmelzwaal are discussing microtubule nucleation in differentiated primary muscle cells.
Primary tasks:
Microscope: inverted Axio Observer 7 SP with Definite Focus 2 (Zeiss, Germany)
Stage: scanning stage 130×100 STEP Set LSM … Read more
Qingyang Liu and Sanne Remmelzwaal are checking the HeLa cells transfected with wild-type Rab8 protein.
Primary tasks: Cell Culture Observation
Microscope: AMG EVOS
Objective:
Epi-fluorescence: GFP, … Read more
Jingchao Wu is studying
Primary tasks: Imaging of fixed samples
Microscope: Nikon Eclipse Ni-E
Objective:
Primary tasks:
3D Single Molecule Localization Microscopy using Adaptive Optics
Microscope: Nikon Eclipse Ti with Perfect Focus System 3
Objectives:
Nikon Apo TIRF 100x/1.49 NA oil
Nikon PLAN FLUOR 10x/0.3 NA air
Camera: 2 x Hamamatsu Flash 4.0v2
Lasers:
Omicron … Read more
Kai Jiang is studying the microtubule minus end proteins in the cells
Primary tasks:
TIRF microscopy, Single Molecule Imaging, FRAP, Photo-Activation, Photo-Ablation (532 nm)
Microscope: Nikon Eclipse Ti with Perfect Focus System
Objective: Apo TIRF 100x / 1.49, oil, WD=0.09-0.16 … Read more
