Equipment

Alfredo Rates Soriano making cells running in circles

Primary tasks:
• Optogenetics
• Long-term imaging
• DIC
• Phase-Contrast

Microscope: Nikon Ti2

Fluorescence Filters: ET-DAPI (49028), ET-Narrow Band EGFP (49020), ET- 514nm for EYFP (49905), ET-mCherry (49008), ET-CY5 (49006) – … Read more

Lasers:

• Insight X3 (680-1300nm, ~2.5W)
• MaiTai HPDS (690-1040nm, ~2W)

Epifluorescence excitation:

• Olympus U-HGLGPS light source

Epifluorescence cubes:

• U-FUW (Ex340-390, DM410, Em420+)
• U-FBN (Ex470-495, DM505, Em510+)
• U-FGWA (Ex530-550, DM570, 575-625)

Objective:

• 25x/1.05 Water (Olympus XLPlan N, WD=2 mm,

Primary tasks:

• Confocal microscopy
• 3D imaging
• Super-resolution imaging

Microscope: Axio Observer 7 with Definite Focus 3

Stage: motorized, Märzhäuser

Piezo-Z: yes, stage

Objectives:
    Plan-Neofluar 5x/0.16 WD=18.5mm, Air
    Plan-Apochromat 20x/0.8 WD=0.55mm, Air
    C-Apochromat 40x/1.20 … Read more

Primary tasks:

• Ring-TIRF
• 3D SIM
• FRAP
• DIC

Excitation lasers:
405 nm Vortran Stradus (100 mw)
445 nm Omicron LuxX (100 mw)
488 nm Coherent (100 mw)
515 nm Coherent (100 mw)
568 nm Coherent (100 mw)
640 nm … Read more

Primary tasks: Cell Culture Observation

Microscope: EVOS M5000

Objective:
     2x LWD, 0.06NA / 5.62WD (AMEP4931)
     4x LWD PH, 0.13NA / 10.58WD (AMEP4980)
     10x LWD PH, 0.30NA / 7.13WD (AMEP4981)
     20x LWD PH, 0.45NA … Read more

Primary tasks: Cell Culture Observation

Microscope: AMEX1000

Objective:
     Achro 4x LWD PH, 0.13NA / 16.9WD (AMEP4932)
     Achro 10x LWD PH, 0.25NA / 6.9WD (AMEP4933)
     Achro 20x LWD PH, 0.40NA / 6.8 WD (AMEP4934)

LED light source … Read more

Primary tasks:

• Ultrafast, high NA, single objective lightsheet volumetric microscopy (SOLM)
• (3D) far-red multi-color single-molecule localization microscopy (STORM)

Microscope base: Nikon Eclipse Ti with Perfect Focus System

Stage: Applied Scientific Instrumentation MS-2000

Heating/CO2: Tokai Hit INUBG2E-ZILCS Stage Top Incubator

Optical … Read more

Primary tasks:

• Confocal live cell imaging
• 3D imaging
• FRAP
• Optogenetics

Microscope: Eclipse Ti2-E with PFS (Nikon, Japan)

Stage: MS-2000-XYZ with Piezo Top Plate (ASI, USA)

Piezo-Z: MS-2000-XYZ with Piezo Top Plate (ASI, USA)

Confocal Spinning Disc Unit: CSU-W1-T1 (Yokogawa, … Read more