Category: Equipment

Zeiss Lattice Lightsheet LL7 pre-serial system


 Malina Iwanski is using LL7 system.

Primary task:  Lattice lightsheet imaging of single cells

Excitation lens:  13.3x / 0.44

Observation lens:  44.83x/1

Light Sheet:  15×550, 15×650

  30×700, 300×1000

  100×1400, 100×1800

Lasers:  488, 561, 640

Software:  … Read more

Posted in BIC, Equipment

Confocal Laser Scanning microscope Carl Zeiss LSM880 Fast AiryScan


Boris Shneyer and Sanne Remmelzwaal are discussing microtubule nucleation in differentiated primary muscle cells.

Primary tasks:

  • Confocal microscopy
  • 3D imaging
  • SuperResolution

Microscope: AxioObserver 7 SP with Definite Focus 2

Spectral detector 3 PMT
Airyscan module GaAsP for LSM 880 with … Read more

Posted in BIC, Equipment

Cell culture digital fluorescent microscope EVOS

Digital inverted fluorescent microscope AMG EVOS
Qingyang Liu and Sanne Remmelzwaal are checking the HeLa cells transfected with wild-type Rab8 protein.

 

Primary tasks: Cell Culture Observation

Microscope: AMG EVOS

Objective:

  • LPlan PH2 4x/0.13 phase-contrast
  • Plan Fluor 10x/0.3
  • Plan Fluor 20x/0.5
  • LPlan PH2 20x/0.40 phase-contrast

Epi-fluorescence:… Read more

Posted in Equipment

Upright FL microscope Nikon Eclipse Ni

Upright fluorescence microscope Nikon Ni-U

Upright FL microscope Nikon Eclipse Ni
Jingchao Wu is studying Golgi Apparatus

 

Primary tasks: Imaging of fixed samples

Microscope: Nikon Eclipse Ni-U

Objective:

  • Plan Apo Lambda 100x N.A. 1.45 oil
  • Plan Apo Lambda 60x N.A. 1.40 oil
  • Plan Fluor 40x
… Read more
Posted in Equipment

Super-Resolution STORM/PALM home made system -2

Super-resolution STORM/PALM home made system (N2)

Super-Resolution STORMPALM home made system -2

Primary tasks:
3D Single Molecule Localization Microscopy using Adaptive Optics

Microscope:
Nikon Eclipse Ti with Perfect Focus System 3

Objectives:
Nikon Apo TIRF 100x N.A. 1.49 oil

Beamsplitter:
Optosplit 3 LS (allows simultaneous three-color … Read more

Posted in Equipment

TIRF 3 Nikon-Roper ILAS-2 PhotoAblation

TIRF Nikon Ti – Roper iLas-2 FRAP/Photoablation system

TIRF 3 Nikon-Roper ILAS-2 PhotoAblation
Kai Jiang is studying the microtubule minus end proteins in the cells

Primary tasks:
TIRF microscopy, Single Molecule Imaging, FRAP, Photo-Activation, Photo-Ablation (532 nm)

Microscope: Nikon Eclipse Ti with Perfect Focus System… Read more

Posted in Equipment

Confocal Leica SP8 FLIM STED3X super-resolution

Super Resolution and confocal microscope Leica TCS SP8 STED3X SMD FLIM

Confocal Leica SP8 FLIM STED3X super-resolution
Joanna Lipka is studying the microtubules in primary hippocampal neurons  

Primary tasks:

  • STED super-resolution imaging
  • Confocal live cell microscopy
  • FLIM
  • FCS

Microscope
Leica DMI 6000 CS AFC with Adaptive … Read more

Posted in Equipment

Confocal Zeiss LSM 700-2

Confocal Laser Scanning microscope Carl Zeiss LSM700-2

Confocal Zeiss LSM 700-2

Maud Martin is studying microtubules in endothelial cells

Primary tasks:

  • Confocal microscopy
  • 3D imaging
  • FRAP

Microscope: AxioObserver Z1

Objectives:

  • Plan-Apochromat 20x/0.8 (WD=0.55mm)
  • EC Plan-Neofluar 40x/1.30 Oil DIC (WD=0.21mm)
  • Plan-Apochromat 63x/1.40 Oil DIC (WD=0.19mm)
… Read more
Posted in Equipment