Plasma Cleaner
Eugene Katrukha and Bas Cloin are cleaning the cover glasses for the single molecule imaging
Primary tasks:
• Cleaning the cover glasses for the single molecule imaging
Model: Harrick Plasma Cleaner PDC-002
Includes a 6″ diameter x 6.5″ length … Read more
Super-Resolution STORM/PALM home made system
Lukas Kapitein is explaining the working principles of the custom-made super-resolution microscope
Primary tasks:
Single Molecule Fluorescence Microscopy, Total Internal Reflection Fluorescence
Microscopy, Super-resolution Microscopy (PALM, STORM), Scanning Stage Microscopy
Microscope: Nikon Eclipse Ti with Perfect Focus System
Objectives:… Read more
Wide-field DIC Phase Contrast FL “PEX”-scope
Sam van Beuningen and Mariella Franker are studying the molecular motors using inducible PEX assay
Primary tasks:
• Long-term imaging
• DIC
• Phase-Contrast
Microscope: Nikon Ti
Fluorescence Filters:
ET-EBFP2 (49021), ET-GFP (49002), ET-mCherry (49008),
ET-Qdot 625 – all … Read more
TIRF 1 Nikon-Roper ILAS
Joanna Lipka is studying AMPA-receptor in hippocampal neurons using FRAP assay
Primary tasks:
TIRF microscopy, Single Molecule Imaging, FRAP, Photo-Activation, Multi-position acquisition,
Electrical Stimulation, Perfusion Experiments, Phase Contrast Time Lapse Microscopy
Microscope: Nikon Eclipse TE2000 with Perfect Focus System… Read more
TIRF 2 Nikon-Roper ILAS PhotoAblation
Andrea Marques and Stine Morthorst are studying the exocytosis of Rab6 vesicles
Primary tasks:
TIRF microscopy, Single Molecule Imaging, FRAP, Photo-Activation, Photo-Ablation (532 nm)
Microscope: Nikon Eclipse Ti with Perfect Focus System
Objective: Nikon Apo TIRF 100x N.A. 1.49 oil… Read more
Spinning Disc 1 Nikon-Roper ILAS PhotoAblation
Microscopy facility manager Ilya Grigoriev, in front of Spinning Disc microscope
Primary tasks:
Confocal Spinning Disc microscopy, 3D imaging, FRAP, Photo-Activation, Photo-Ablation (355 nm)
Microscope: Nikon Eclipse Ti with Perfect Focus System
Confocal Head: Yokogawa CSU-X1-A1
Objective:
Apo TIRF 100x … Read more
Two photon Femtonics 2D-galvo microscope
Primary tasks:
Two-photon microscopy, calcium imaging
in combination with single-cell electroporation and/or electrophysiology
Microscope:
Femtonics 2D upright microscope (based on Olympus BX61 WI microscope)
Objectives:
Nikon CFI Apochromat 60X NIR water immersion
Laser:
SpectraphysicsMaiTai HP ultrafast femtosecond laser
Tuning range … Read more
Confocal Zeiss LSM 700
Marijn Kuijpers and Laura Gumy are studying microtubules in the growth cone during axon regeneration
Primary tasks:
- Confocal microscopy
- 3D imaging
- FRAP
Microscope: AxioObserver Z1
Objectives:
Plan-Apochromat 20x/0.8 (WD=0.55mm)
EC Plan-Neofluar 40x/1.30 Oil DIC (WD=0.21mm)
Plan-Apochromat 63x/1.40 Oil DIC … Read more