Category: Equipment

Plasma Cleaner

Plasma Cleaner 1

Plasma Cleaner 2
Eugene Katrukha and Bas Cloin are cleaning the cover glasses for the single molecule imaging

 

Primary tasks:
• Cleaning the cover glasses for the single molecule imaging

Model: Harrick Plasma Cleaner PDC-002

Includes a 6″ diameter x 6.5″ length … Read more

Posted in BIC, Equipment

Super-Resolution STORM/PALM home made system

Super-resolution STORM-PALM home made system
Lukas Kapitein is explaining the working principles of the custom-made super-resolution microscope

 

Primary tasks:
Single Molecule Fluorescence Microscopy, Total Internal Reflection Fluorescence
Microscopy, Super-resolution Microscopy (PALM, STORM), Scanning Stage Microscopy

Microscope: Nikon Eclipse Ti with Perfect Focus System

Objectives:Read more

Posted in BIC, Equipment

Wide-field DIC Phase Contrast FL “PEX”-scope

Wide-field Nikon Ti PEXscope 1

Wide-field Nikon Ti PEXscope 2
Sam van Beuningen and Mariella Franker are studying the molecular motors using inducible PEX assay

 

Primary tasks:
• Long-term imaging
• DIC
• Phase-Contrast

Microscope: Nikon Ti

Fluorescence Filters:
ET-EBFP2 (49021), ET-GFP (49002), ET-mCherry (49008),
ET-Qdot 625 – all … Read more

Posted in BIC, Equipment

TIRF 1 Nikon-Roper ILAS

TIRF Nikon TE2000 Roper FRAP system
Joanna Lipka is studying AMPA-receptor in hippocampal neurons using FRAP assay

 

Primary tasks:
TIRF microscopy, Single Molecule Imaging, FRAP, Photo-Activation, Multi-position acquisition,
Electrical Stimulation, Perfusion Experiments, Phase Contrast Time Lapse Microscopy

Microscope: Nikon Eclipse TE2000 with Perfect Focus System… Read more

Posted in BIC, Equipment

TIRF 2 Nikon-Roper ILAS PhotoAblation

TIRF Nikon Ti Roper iLas FRAPPhotoablation system
Andrea Marques and Stine Morthorst are studying the exocytosis of Rab6 vesicles


Primary tasks:
TIRF microscopy, Single Molecule Imaging, FRAP, Photo-Activation, Photo-Ablation (532 nm)

Microscope: Nikon Eclipse Ti with Perfect Focus System

Objective: Nikon Apo TIRF 100x N.A. 1.49 oil… Read more

Posted in BIC, Equipment

Spinning Disc 1 Nikon-Roper ILAS PhotoAblation

Confocal Yokogawa Spinning Disc X1 Nikon Ti Roper iLas FRAPPhotoablation system
Microscopy facility manager Ilya Grigoriev, in front of Spinning Disc microscope

Primary tasks:
Confocal Spinning Disc microscopy, 3D imaging, FRAP, Photo-Activation, Photo-Ablation (355 nm)

Microscope: Nikon Eclipse Ti with Perfect Focus System

Confocal Head: Yokogawa CSU-X1-A1

Objective:
Apo TIRF 100x … Read more

Posted in BIC, Equipment

Two photon Femtonics 2D-galvo microscope

Confocal 2-Photon microscope Femtonics

Primary tasks:
Two-photon microscopy, calcium imaging
in combination with single-cell electroporation and/or electrophysiology

Microscope:
Femtonics 2D upright microscope (based on Olympus BX61 WI microscope)

Objectives:
Nikon CFI Apochromat 60X NIR water immersion

Laser:
SpectraphysicsMaiTai HP ultrafast femtosecond laser
Tuning range … Read more

Posted in Equipment

Confocal Zeiss LSM 700

Confocal Laser Scanning microscope Carl Zeiss LSM700 1

Confocal Laser Scanning microscope Carl Zeiss LSM700 2
Marijn Kuijpers and Laura Gumy are studying microtubules in the growth cone during axon regeneration

 

Primary tasks:

  • Confocal microscopy
  • 3D imaging
  • FRAP

Microscope: AxioObserver Z1

Objectives:

Plan-Apochromat 20x/0.8 (WD=0.55mm)
EC Plan-Neofluar 40x/1.30 Oil DIC (WD=0.21mm)
Plan-Apochromat 63x/1.40 Oil DIC … Read more

Posted in BIC, Equipment