Category: BIC

Re-scan Confocal microscope RCM1

 

Primary tasks:

  • Laser scanning confocal (automated super resolution)
  • Widefield fluorescence microscopy
  • 3D imaging

Microscope: Nikon Ti-U

Stage: TI-S-E Motorized stage

Objectives:
     Plan fluor 10x / 0.30 (Nikon, Japan) 867 nm/pix
     Plan fluor 20x / 0.50 (Nikon, Japan)Read more

Posted in BIC, Equipment

Zeiss Apotome 3 optical sectioning microscope

 

Primary tasks:

  • 3D imaging

Microscope: Axio Imager Z2 (upright)

Stage: motorized (Märzhäuser, Germany)

Objectives:
     Plan-Neofluar 10x/0.3 WD=5.2mm, Air
     Plan-Apochromat 20x/0.8 WD=0.55mm, Air
     C-Apochromat 40x/1.20 WD=0.28mm, Water Immersion
     Plan-Apochromat 40x/1.3 WD=0.21mm, Oil Immersion (Zeiss, Germany)
     … Read more

Posted in BIC, Equipment

Confocal Laser Scanning microscope Zeiss LSM980 AiryScan 2

 

Primary tasks:

  • Confocal microscopy
  • 3D imaging
  • Super-resolution imaging

Microscope: Axio Observer 7 SP with Definite Focus 3

Stage: motorized, Märzhäuser

Piezo-Z: yes, stage

Objectives:
    Plan-Neofluar 5x/0.16 WD=18.5mm, Air
    Plan-Apochromat 20x/0.8 WD=0.55mm, Air
    C-Apochromat … Read more

Posted in BIC, Equipment

Confocal Laser Scanning microscope Zeiss LSM900

 

Primary tasks:

  • Confocal microscopy
  • 3D imaging
  • FRAP

Microscope: upright Axio Imager Z2 (Zeiss, Germany)

Stage: Scanning stage 130×85 STEP Set LSM (Märzhäuser, Germany)

Objectives:
     EC Plan-Neofluar 10x/0.3  WD=5.2mm, Air (Zeiss, Germany)
     Plan-Apochromat 20x/0.8 WD=0.55mm, Air (Zeiss, Germany)Read more

Posted in BIC, Equipment

Andor benchtop confocal BC43

 

Primary task: Long-term spinning disk confocal imaging of living cells and organoids.

Illumination: Brightfield and differential phase contrast transmitted white light, wide-field epifluorescence, Borealis™-illuminated multipoint confocal with 405/488/561/638 nm excitation lasers.

Objectives:
2x Plan UW Air … Read more

Posted in BIC, Equipment

Zeiss Lattice Lightsheet LL7 system


 Malina Iwanski is using LL7 system.

Primary task:  Lattice lightsheet imaging of single cells

Excitation lens:  13.3x / 0.44

Observation lens:  44.83x/1

Light Sheet:  15×550, 15×650

  30×700, 300×1000

  100×1400, 100×1800

Lasers:  488, 561, 640

Cameras:  Two … Read more

Posted in BIC, Equipment

Confocal Laser Scanning microscope Zeiss LSM880 Fast AiryScan


Boris Shneyer and Sanne Remmelzwaal are discussing microtubule nucleation in differentiated primary muscle cells.

Primary tasks:

  • Confocal microscopy
  • 3D imaging
  • SuperResolution

Microscope: inverted Axio Observer 7 SP with Definite Focus 2 (Zeiss, Germany)

Stage: scanning stage 130×100 STEP Set LSM … Read more

Posted in BIC, Equipment

Testing ZEISS Celldiscoverer 7

2018.11.22

Testing ZEISS Celldiscoverer 7

 

 

 

Zeiss, Breda… Read more

Posted in Activities, BIC