Two photon Femtonics 2D-galvo microscope

Primary tasks:
Two-photon microscopy, calcium imaging
in combination with single-cell electroporation and/or electrophysiology

Microscope:
Femtonics 2D upright microscope (based on Olympus BX61 WI microscope)

Objectives:
Nikon CFI Apochromat 60X NIR water immersion

Laser:
SpectraphysicsMaiTai HP ultrafast femtosecond laser
Tuning range 690-1040 nm, pulse width <100 fs, 80 MHz repetition rate

Scanner:
Fast galvanoscanners with digital servo drivers

Detectors:
2 sub-stage (transmitted) and 2 upper (reflected) PMTs for high sensitivity
HamamatsuGaAs photomultiplier tubes (PMTs)
1 transmitted IR detector for recognizing of unstained objects (e.g. cell bodies, pipette, stimulator)

Beamsplitter(in front of PMTs):
Green PMT detection band: 475-575 nm
Red PMT detection band: 600-675 nm

Stage:
Luigs&Neumann 380FM-2P (computer-controlled) + keypad SM-5

Optical Table:
TMC

Software:
Femtonics modular measurement control and analysis software “MES” (Matlab-based)

Other equipment:
Luigs&Neumann micromanipulator UNIT Junior LE with controller SM7 for 5 axes
Axon Axopatch 200B patch clamp amplifier
Axoporator 800A single-cell electroporator
Narishige pipette puller PC-10

Location: Z-512
Contact Person: Corette Wierenga