Qingyang Liu and Sanne Remmelzwaal are checking the HeLa cells transfected with wild-type Rab8 protein.

Primary tasks: Cell Culture Observation

Microscope: AMG EVOS

Objective:

• LPlan PH2 4x/0.13 phase-contrast
• Plan Fluor 10x/0.3
• Plan Fluor 20x/0.5
• LPlan PH2 20x/0.40 phase-contrast

Epi-fluorescence:… Read more

Upright fluorescence microscope Nikon Ni-U

Jingchao Wu is studying Golgi Apparatus

Primary tasks: Imaging of fixed samples

Microscope: Nikon Eclipse Ni-U

Objective:

• Plan Apo Lambda 100x N.A. 1.45 oil
• Plan Apo Lambda 60x N.A. 1.40 oil
• Plan Fluor 40x

Super-resolution STORM/PALM home made system (N2)

Primary tasks:
3D Single Molecule Localization Microscopy using Adaptive Optics

Microscope:
Nikon Eclipse Ti with Perfect Focus System 3

Objectives:
Nikon Apo TIRF 100x N.A. 1.49 oil

Beamsplitter:
Optosplit 3 LS (allows simultaneous three-color … Read more

TIRF Nikon Ti – Roper iLas-2 FRAP/Photoablation system

Kai Jiang is studying the microtubule minus end proteins in the cells

Primary tasks:
TIRF microscopy, Single Molecule Imaging, FRAP, Photo-Activation, Photo-Ablation (532 nm)

Microscope: Nikon Eclipse Ti with Perfect Focus System… Read more

Super Resolution and confocal microscope Leica TCS SP8 STED3X SMD FLIM

Joanna Lipka is studying the microtubules in primary hippocampal neurons  

Primary tasks:

• STED super-resolution imaging
• Confocal live cell microscopy
• FLIM
• FCS

Microscope
Leica DMI 6000 CS AFC with Adaptive … Read more

Confocal Laser Scanning microscope Carl Zeiss LSM700-2

Maud Martin is studying microtubules in endothelial cells

Primary tasks:

• Confocal microscopy
• 3D imaging
• FRAP

Microscope: AxioObserver Z1

Objectives:

• Plan-Apochromat 20x/0.8 (WD=0.55mm)
• EC Plan-Neofluar 40x/1.30 Oil DIC (WD=0.21mm)
• Plan-Apochromat 63x/1.40 Oil DIC (WD=0.19mm)