Plasma Cleaner
Eugene Katrukha and Bas Cloin are cleaning the cover glasses for the single molecule imaging
Primary tasks:
• Cleaning the cover glasses for the single molecule imaging
Model: Harrick Plasma Cleaner PDC-002
Includes a 6″ diameter x 6.5″ length Pyrex … Read more
Super-Resolution STORM/PALM home made system
Lukas Kapitein is explaining the working principles of the custom-made super-resolution microscope
Primary tasks:
Single Molecule Fluorescence Microscopy, Total Internal Reflection Fluorescence
Microscopy, Super-resolution Microscopy (PALM, STORM), Scanning Stage Microscopy
Microscope: Nikon Eclipse Ti with Perfect Focus System
Objectives:… Read more
Wide-field Nikon Ti “PEX”-scope
Sam van Beuningen and Mariella Franker are studying the molecular motors using inducible PEX assay
Primary tasks:
• Long-term imaging
• DIC
• Phase-Contrast
Microscope: Nikon Ti
Fluorescence Filters: ET-EBFP2 (49021), ET-GFP (49002), ET-mCherry (49008), ET-Qdot 625 – all Chroma… Read more
TIRF 1 Nikon-Roper ILAS
Joanna Lipka is studying AMPA-receptor in hippocampal neurons using FRAP assay
Primary tasks:
TIRF microscopy, Single Molecule Imaging, FRAP, Photo-Activation, Multi-position acquisition,
Electrical Stimulation, Perfusion Experiments, Phase Contrast Time Lapse Microscopy
Microscope: Nikon Eclipse TE2000 with Perfect Focus System… Read more
TIRF Nikon Ti – Roper iLas FRAP/Photoablation system
Andrea Marques and Stine Morthorst are studying the exocytosis of Rab6 vesicles
Primary tasks:
TIRF microscopy, Single Molecule Imaging, FRAP, Photo-Activation, Photo-Ablation (532 nm)
Microscope: Nikon Eclipse Ti with Perfect Focus System
Objective: Apo TIRF 100x / 1.49, oil, WD=0.09-0.16 … Read more
Confocal Spinning Disc 1 Nikon Ti – Yokogawa X1 – Gataca iLAS FRAP/Photoablation system
Microscopy facility manager Ilya Grigoriev, in front of Spinning Disc microscope
Primary tasks:
- Confocal Spinning Disc microscopy
- 3D imaging
- FRAP
- Photo-Activation
- Photo-Ablation (355 nm)
Microscope: Eclipse Ti-E with PFS (Nikon, Japan)
Stage: MS-2000-XYZ with Piezo Top Plate (ASI, USA)
Piezo-Z: … Read more
Two photon Femtonics 2D-galvo microscope
Primary tasks:
Two-photon microscopy, calcium imaging
in combination with single-cell electroporation and/or electrophysiology
Microscope:
Femtonics 2D upright microscope (based on Olympus BX61 WI microscope)
Objectives:
Nikon CFI Apochromat 60X NIR water immersion
Laser:
SpectraphysicsMaiTai HP ultrafast femtosecond laser
Tuning range … Read more
Confocal Laser Scanning microscope Zeiss LSM700
Marijn Kuijpers and Laura Gumy are studying microtubules in the growth cone during axon regeneration
Primary tasks:
- Confocal microscopy
- 3D imaging
- FRAP
Microscope: AxioObserver Z1
Objectives:
EC Plan-Neofluar 10x/0.3 (WD=5.2mm)
Plan-Apochromat 20x/0.5 (WD=2mm)
EC Plan-Neofluar 40x/1.30 … Read more